YMC-Triart C18


Long column lifetime under harsh chemical conditions

YMC-Triart C18 offers outstanding chemical and physical durability over a wide pH range at high temperatures.
As erythromycins are known to be easily degraded under acidic (pH < 6.5) conditions, higher pH is preferable. In addition, higher temperatures tend to show better peak shape. The enhanced chemical durability of Triart C18 enables highly reproducible analysis at high pH and high temperature.

Analysis of erythromycins at pH 7.9, 70ºC

1.  Erythromycin
2.  Erythromycin ethylsuccinate
3.  Erythromycin estolate

Column YMC-Triart C18 (3 μm, 120Å), 50 X 2.0 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH 7.9)/acetonitrile/methanol (40/45/15)
Flow rate 0.2 mL/min
Temperature 70ºC
Detection UV at 210 nm

Effective for analysis of highly polar compounds using 100% aqueous conditions

Using 100% aqueous mobile phase with conventional C18 columns generally shows poor performance (retention and peak shape) owing to low surface hydration caused by repulsion between aqueous mobile phase and the hydrophobic bonded phase. Triart C18 is designed to retain both moderate hydrogen bonding capacity and hydrophobicity on the surface by optimizing the bonding density of the C18 phase. Its versatility makes it ideal for a first choice ODS column, and is also applicable to the analysis of polar compounds under 100% aqueous mobile phase conditions.
In the experiment illustrated here, Triart C18 shows stable retention of organic acids, even after stopping the pump.

1.  Oxalic acid
2.  Tartaric acid 
3.  Glycolic acid
4.  Formic acid
5.  L-Malic acid
6.  Malonic acid
7.  Lactic acid

  8.   Acetic acid
  9.   Maleic acid
10 .  Citric acid
11 .  Succinic acid
12 .  Fumaric acid
13 .  Acrylic acid
14 .  Propionic acid

Column YMC-Triart C18 (3 μm, 120 Å), 150 X 3.0 mmI.D.
Eluent 20 mM H3PO4
Flow rate 0.425 mL/min
Temperature 37ºC
Detection UV at 220 nm

Minimizing strong solvent/sample loading effects

YMC-Triart C18 can tolerate larger injection volumes of samples containing solvents that have a strong eluting ability (e.g., acetonitrile), while giving better peak shapes than conventional columns. This can be important for samples pre-treated with higher concentrations of organic solvent, crude reaction samples, and samples with poor solubility.

Influence of injection volume on peak shape

Column 5 μm, 50 X 2.0 mmI.D. or 2.1 mmI.D.
Eluent A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-0.5 min), 5-100%B (0.5-2.5 min)
Flow rate 0.4 mL/min
Temperature 40ºC
Detection UV at 275 nm

Ideal for high-sensitivity analysis using LC/MS

On the Triart C18 column, very low levels of bleeding (leaching) are achieved due to improvements of the production procedure and product stability. The background noise of Triart C18 on LC/MS (TIC) is almost the same as that of a blank run with no column. These results prove that there is little bleeding from the Triart C18 column. Very low background noise and a high S/N ratio are expected on Triart columns, even with high-sensitivity detectors.

Bleed test by LC/MS

Column 5 µm, 50 X 2.0 mmI.D.
Eluent A) water/formic acid (100/0.1)
B) acetonitrile/formic acid (100/0.1)
5%B (0-1 min), 5-100%B (1-5 min), 100%B (5-10 min), 100-5%B (10-10.1 min), 5%B (10.1-12.5 min)
Flow rate 0.4 mL/min
Temperature 40ºC
Detection ESI positive, TIC (Mass Range: 50-1000)

For separation of biomolecules with molecular weight of up to 30,000 using high temperature

Changing the column temperature will provide selectivity change as well as peak shape improvement. YMC-Triart C18, which offers excellent durability even under an elevated temperature condition, is effective for the separation of peptides and proteins.

  1. Leu-Enkephalin    (MW 556)
  2. Oxytocin        (MW 1,007)
  3. β-Endorphin       (MW 3,465)
  4. Insulin         (MW 5,733)
  5. β-Lactoglobulin A (MW 18,400)
Column YMC-Triart C18 (1.9 μm, 120 Å), 50 X 2.0 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
10-80%B (0-5 min)
Flow rate 0.4 mL/min
Detection 220 nm