Size exclusion chromatography column
YMC-Pack Diol

YMC-Pack Diol is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to 1,000,000.
Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.


Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000
  • Utilizes silica gel base with high resistance to pressure
  • Low-cost gel filtration column
  • Useful for molecular weight determination of proteins and sugars
  • Smooth method transfer from HPLC to UHPLC achieved by identical selectivity across particle sizes
  • Minimal secondary interactions

Application data