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Columns

Size exclusion chromatography column
YMC-Pack Diol

Features

Calibration curves of various proteins

Comparison with different pore sizes


Diol-120, Diol-200, and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.

Column YMC-Pack Diol 5 µm, 300 X 8.0 mmI.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

Comparison with different particle sizes

YMC-Pack Diol column has an identical calibration curve across all of the particle sizes including 2 µm. This would realize smooth method transfer between HPLC and UHPLC.

Column YMC-Pack Diol, 300 X 4.6 mmI.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25°C
Detection UV at 280 nm

Analysis of plasma contituents

Eluent 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl
Flow rate 0.5 mL/min
Temperature ambient (25°C)
Detection UV at 280 nm

Excellent durability

YMC-Pack Diol column maintained its performance for more than 500 injections on monoclonal antibody analysis.

Column YMC-Pack Diol-300 2 µm
150 X 4.6 mmI.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0)
containing 0.2 M NaCl
Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm

For separation of peptides with molecular weights of 10,000 or less

Column YMC-Pack Diol-60 5 µm, 500 X 8.0 mmI.D.
Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0)
containing 0.2 M NaCl/acetonitrile (70/30)
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 215 nm

For molecular weight determination of sugars

For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds, Diol-60, Diol-120, Diol-200 and Diol-300 are useful individually or in combination.

Column YMC-Pack Diol 5 µm, 500 X 8.0 mmI.D.
Eluent water
Flow rate 1.0 mL/min
Temperature ambient
Detection RI

Excellent reproducibility

YMC-Pack Diol column has excellent lot-to-lot reproducibility. This feature is ideal for quality control of protein-based drugs including monoclonal antibodies.

1. Thyroglobulin
2. γ-Globulin
3. Ovalbumin
4. Myoglobin
5. Vitamin B12

Column YMC-Pack Diol-300 5 µm, 300 X 8.0 mmI.D.
Eluent 0.1 M NaH2PO4-Na2HPO4 (pH 7.0)
containing 0.2 M NaCl
Flow rate 1.0 mL/min
Temperature ambient
Detection UV at 280 nm
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