Columns
YMC-Triart PFP is a pentafluorophenyl bonded phase. The selectivity is unique owing to the various forces arising from hydrophobic, π–π, and dipole–dipole interactions.
YMC-Triart PFP is especially effective for improving the separation of aromatic compounds, nitro compounds, and halogen compounds because the selectivity is very different from that of other columns.
| SPECIFICATION | |
| Base | organic/inorganic hybrid silica |
|---|---|
| Particle size | 1.9 µm, 3 µm, 5 µm |
| Pore size | 120 Å |
| Carbon contents | 15% |
| pH range | 1-8 |
As the differences in the hydrophobicities of the cis-trans isomers of piperine, which is a pungent component of pepper, are small, commonly used reversed phase columns are not suitable for their separation. However, YMC-Triart PFP provides excellent separations as it recognizes minor charge localizations in a molecule owing to various binding through as π–π and dipole–dipole interactions. YMC-Triart PFP shows high selectivity towards compounds with small structural differences.
| Column | 5 µm, 150 X 3.0 or 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/0.1% formic acid (40/60) |
| Flow rate | 0.425 mL/min for 3.0 mmI.D. 1.0 mL/min for 4.6 mmI.D. |
| Temperature | 25°C |
| Detection | UV at 280 nm |
