Columns
YMC-Triart Diol-HILIC is a HILIC (hydrophilic interaction chromatography) column based on an organic/inorganic hybrid particle synthesized with dihydroxypropyl groups. Triart Diol-HILIC is ideal for a separation of polar and hydrophilic compounds that are not retained by reversed-phase (C18, C8, and others) chromatography. Triart Diol-HILIC begins with an organic/inorganic hybrid-based particle with excellent durability and usability across a wide pH range. Excellent reproducibility is achieved by the use of the dihydroxypropyl group–a neutral functional group exhibiting low nonspecific adsorption.
| SPECIFICATION | |
| Base | organic/inorganic hybrid silica |
|---|---|
| Particle size | 1.9 µm, 3 µm, 5 µm |
| Pore size | 120 Å |
| pH range | 2-10 |
Triart C18 (reversed-phase) shows very weak retention and poor resolution of L-ascorbic acid and its stereoisomer (erythorbic acid), even with a 100% aqueous mobile phase. However, Triart Diol-HILIC shows strong retention and better resolution of these compounds with a mobile phase containing 90% organic solvent.
| Column | 5 µm, 150 X 3.0 mmI.D. |
|---|---|
| Flow rate | 0.425 mL/min |
| Temperature | 40°C |
| Detection | UV at 254 nm |
| Injection | 4 µL (0.05 mg/mL) |
Triart Diol-HILIC provides highly reproducible separations, even at high pH and high temperatures. The lifetime of Triart Diol-HILIC is much greater than that of conventional silica-based diol columns.
Durability in high pH (pH 11, 50°C)
| Column | 5 µm, 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water/NH3 (90/10/0.1) pH 11.3 |
| Flow rate | 1.0 mL/min |
| Temperature | 50°C |
| Sample | Cytosine |
