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YMC-Pack Pro C8&C4

All phases in the Pro-family are ideal for the analysis of all types of organic molecules but specifically basic and metal chelating compounds. The ultrapure silica and very low residual silanol activity means that these columns frequently do not require the use of ion-pairing reagents or ion-suppression techniques, making them ideal for LC/MS applications.

SPECIFICATION

Particle size : 3µm, 5µm
Pore size : 120Å
Carbon contents : C8; 10%
 C4; 7%
Usable pH range : 2.0-7.5

POINT

  • Consistently reproducible quality
  • Documentation of each manufacturing step
  • Consistently high packing quality + column efficiency
  • Stringent specifications
  • Individual test of each column(test chromatogram)
  • Batch identification for each column


Separation of basic compounds

Separation of basic compounds

  1. Scoporamine bromide
  2. Atropine sulfate
  3. Cinchonin
  4. Quinine
  5. Papaverine chloride
Column : YMC-Pack Pro C8 (5µm, 120Å)
 150 x 4.6mmI.D.
Eluent : acetonitrile/20mM KH2PO4 (20/80)
Flow rate : 1.0 mL/min
Temperature : 30ºC
Detection : UV at 210 nm

Effectively endcapped Pro C8 and C4 offer better peak shape for separation of basic compounds such as alkaloids.

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Short time analysis

Antipyretic analgesic

Optimization of separation by Pro C8 and C4

  1. Caffeine
  2. Ethenzamide
  3. Bromovalerylurea
  4. Ibuprofen
Column : 150 x 4.6mmI.D.
Eluent : methanol/20mM H3PO4
 (60/40)
Flow rate : 1.0 mL/min
Temperature : 37ºC
Detection : UV at 254 nm

This figure shows that Pro C8 and C4 columns allow us to minimize total analysis time while maintaining excellent separation for caffeine and ethenzamide and bromovalerylurea.

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Optimization of separation by Pro C8 and C4

Pro C18

Pro C8&C4

In the case that it is difficult to optimize resolution and analysis time on separation by C18, C8 and C4 columns can be effective alternatives.

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