Chiral separation columns / packing materials


Method scouting

Although chiral chromatography is an effective means of measuring optical purity and optical resolution, suitable column and suitable mobile phase are hard to predict. Method scouting to determine the separation conditions and column can shorten the consideration time. CHIRAL ART, which includes seven column product lineups (five immobilized type and two coated type) can further accelerate establishment of a chromatography method.

Column 3 µm, 75 X 3.0 mmI.D.
Gradient 5-45% (0-6 min), 45%B (6-10 min)
Flow rate 0.425 mL/min
Temperature 25°C
Detection UV at 270 nm
Injection 3 µL (0.5 mg/mL)

Excellent peak shape

CHIRAL ART provide good peak shapes for ionic and metal coordination compounds.

Column 5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol/diethylamine
Flow rate 1.0 mL/min
Temperature 25°C
Detection 230 nm
Injection 10 µL (0.1 mg/mL)

Extended packing durability

CHIRAL ART have outstanding packed bed stability provided by the use of high-strength super-wide-pore silica and innovative packing technology. The column efficiency and selectivity are maintained, even after sequential gradient tests at a high flow rate (three times higher than the normal flow rate) and a high pressure (rapid pressure change).
CHIRAL ART are suitable for the case where one intends to shorten an analysis time, (re-)equilibration time, and/or column cleaning time by increasing the flow rate. This feature is also effective when a highly viscous solvent needs to be used as a mobile phase on immobilized type columns.

Sequential gradient test
Column 5 µm, 250 X 4.6 mmI.D.
Eluent A) n-hexane,
B) ethanol
0-100%B (0-15 min)
Flow rate 3.0 mL/min
Pressure 10-30 MPa/run
Temperature 37°C
Column performance test
Column 5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/ethanol
Flow rate 1.0 mL/min
Temperature 37°C
Detection UV at 230 nm
Sample trans-Stilbene oxide