CHIRAL ART Coated type


Excellent peak shape

CHIRAL ART provide good peak shapes on ionic and metal coordination compounds.

Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol/diethylamine
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm
Injection 10 μL (0.1 mg/mL)

Extended packing durability

CHIRAL ART have outstanding packed bed stability provided by the use of high-strength super-wide-pore silica and innovative packing technology. The column efficiency and selectivity are maintained, even after sequential gradient tests at a high flow rate (three times higher than the normal flow rate) and a high pressure (rapid pressure change).
CHIRAL ART are suitable for the case where one intends to shorten an analysis time, (re-)equilibration time, and/or column cleaning time by increasing the flow rate. This feature is also effective when a highly viscous solvent needs to be used as a mobile phase on immobilized type columns.

Sequential gradient test
Column 5 μm, 250 X 4.6 mmI.D.
Eluent A) n-hexane,
B) ethanol
0-100%B (0-15 min)
Flow rate 3.0 mL/min
Pressure 10-30 MPa/run
Temperature 37℃
Column performance test
Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/ethanol
Flow rate 1.0 mL/min
Temperature 37℃
Detection UV at 230 nm
Sample trans-Stilbene oxide

Effective for preparative separation of enantiomers

On both analytical and preparative separation, separation behavior of CHIRAL ART is equivalent to that of the competitor’s product.

Column 5 μm, 250 X 4.6 mmI.D.
Eluent n-hexane/ethanol (90/10)
Flow rate 1.0 mL/min

High-purity purification utilizing recycling preparation

Ibuprofen enantiomers were purified by utilizing the recycling mode of a multiple preparative HPLC system, LC-Forte/R. Recycling chromatography is effective when method optimization for chiral isolation is difficult using single column separation. The recycling method offers high-purity and high-recovery purification. Furthermore, no solvent is consumed during recycling, which greatly contributes to the reduction of solvent consumption during purification.

Column CHIRAL ART Cellulose-C 10 µm, 250 X 20 mmI.D.
Flow rate 20 mL/min
Detection UV at 265 nm
Injection 5 mL (10 mg/mL)
  Single Column Recycling
Enantiomeric purity (%ee )
   Fr.1 (R)(- -)-Ibuprofen
   Fr.2 (S)(- +)-Ibuprofen
Yield (%) 84 95
Solvent consumption
(mL solvent/g product)
9,523 1,276