Bulk packings

Macro-porous ion exchange media
MacroSep IEX Q

MacroSep IEX Q are innovative strong anion exchange media to target large biomolecules and particles such as adeno-associated viruses (AAVs), protein complexes, and plasmid DNA (pDNA). This media offers an optimized macro-porous structure that ensures efficient purification of these molecules. With its high binding capacity for large particles and molecules in addition to high separation efficiency even at elevated flow rates, MacroSep IEX Q is an ideal solution for downstream processing (DSP) platforms, enabling successful and productive purification of large biotherapeutics.

MacroSep IEX Q

BioPro IEX SmartSep Q30

Specifications
Matrix Hydrophilic methacrylate polymer
Particle size (µm) 30
Pore size (Å) 9000
Charged group -R-N+(CH3)3
Ion-exchange capacity
(meq/mL-resin)
>0.08
Dynamic binding capacity (DBC)
(mg/mL-resin)
30 (Thyroglobulin)
Usable pH range 2-12
Features

High resolution even at a high flow rate

The direct comparison of MacroSep IEX Q with conventional media at two different flow rates clearly shows that MacroSep IEX Q provides excellent resolution, even at high flow rates. This leads to high productivity.

Column 50 X 5.0 mmI.D.
Eluent A) 20 mM Bis-tris propane-HCl (pH 9.0)
B) 20 mM Bis-tris propane-HCl containing 0.5 M TMAC* (pH 9.0)
Gradient 10-50%B (45 CV)
Temperature 25°C
Detection FLS at Ex. 280 nm, Em. 348 nm
Injection 30 µL
Sample AAV2 (1.07 X 1011 vg/mL)

* tetramethylammonium chloride
This research was supported by AMED under Grant Number JP18ae0201001.

Highly efficient purification of AAV2 with MacroSep IEX Q

AAV Full/Empty capsids separation was performed with MacroSep IEX Q. Purity of the full AAV2 after the chromatographic purification was reached to 91.2% against 54.4% of a feed solution, determined by analytical ultracentrifugation (AUC). MacroSep IEX Q is suitable for chromatographic purification of AAV.

Column MacroSep IEX Q (30 µm), 50 X 5.0 mmI.D.
Eluent A) 20 mM Bis-tris propane-HCl (pH 9.0), 1% sucrose,
     0.1% poloxamer 188
B) 20 mM Bis-tris propane-HCl containing
     0.5 M CC* (pH 9.0), 1% sucrose,
     0.1% poloxamer 188
Gradient 0-60%B (36 CV)
Flow rate 1.0 mL/min
Temperature ambient (25°C)
Detection UV at 280 nm
Injection 48 mL
Sample AAV2 (0.72 X 1011 vg/mL)

* choline chloride
This research was supported by AMED under Grant Number JP18ae0201001.

The separation performance for topological isomers of pDNA

MacroSep IEX Q can separate supercoiled (SC) from the non-functional linearized (L) and nicked open-circular (OC) species with higher resolution while conventional media gives poor resolution of those species. MacroSep IEX offers increased productivity with higher purity on pDNA purification.

Column 50 X 5.0 mmI.D.
Eluent A) 20 mM Tris-HCl (pH 8.5)
B) 20 mM Tris-HCl (pH 8.5) containing 1.0 M NaCl
Gradient [MacroSep IEX Q]
82%B (0-1.5 min), 82-86%B (1.5-22.5 min)
[Brand B]
77%B (0-1.5 min), 77-81%B (1.5-22.5 min)
Flow rate 0.4 mL/min
Temperature 25°C
Detection UV at 260 nm
Injection 10 µL (60 µg/mL)
Sample [SC] pUC19 plasmid extracted from E. coli (2686 bp)
[OC] pUC19 plasmid digested with nicking endonuclease Nt.BspQΙ
[L]   pUC19 plasmid digested with restriction enzyme BamHΙ

High dynamic binding capacity (DBC) and high recovery of pDNA

MacroSep IEX Q is designed to have higher DBC value of large biomolecules than conventional media for improving productivity. DBC value of pDNA with each MacroSep IEX Q and conventional media is determined as an example of a large-sized modality. MacroSep IEX Q shows higher DBC with high recovery, compared to the conventional media.

Breakthrough curves
Column MacroSep IEX Q, 26 X 7 mmI.D. (1 mL)
Brand A, 20 X 8 mmI.D. (1 mL)
Equilibration buffer 100 mM CH3COONa-CH3COOH (pH 5.0)
Elution buffer Equilibration buffer containing 1 M NaCl
Flow rate 0.5 mL/min (Residence time 2 min)
Temperature

ambient

Detection UV at 260 nm
Sample

pRP[Exp]-Puro-TRE3G>EGFP (4934 bp)

Sample Concentration 0.2 mg/ml pDNA in Equilibration buffer
containing 0.15 M NaCl
Comparisons of DBC and recovery for pDNA
  DBC
(mg/mL-resin, 10% breakthrough)
Recovery*
(%)

MacroSep IEX Q

7.5 101

Brand A

1.3 101

*Recovery: (Eluted amount/Adsorption amount) X 100