Columns
Pro C18 RS has excellent peak shape for the separation of basic compounds because of high quality end capping. Pro C18 RS has high recognition of stereoselectivity because of high carbon polymeric type bonding.
Pro C18 RS can be used over a wide pH range (1-10) to aid in method development.
| SPECIFICATION | |
| Particle size | 3 µm, 5 µm |
|---|---|
| Pore size | 80 Å |
| Carbon contents | 22% |
| Usable pH range | 1-10 |
pH 1
Pro C18 RS hardly change hydorophobicity of packing material in pH1 even 70°C (column temperature)
Change of hydorophobicity of material in 70°C after the replacement of the solvent in the column with acetonitrile/water/TFA (10/90/1)
pH 10
You can use RS in alkaline eluent in which you can not use general ODS column
Continuous analysis with 20mM H3BO3-NaOH (pH 9.8)/methanol (50/50)
Separation behavior is influenced by changing the pH of the eluent. Pro C18 RS can operate over a wide pH range to allow manipulation of selectivity and retention.
| Column | YMC-Pack Pro C18 RS (5 µm, 80 Å) 150 X 4.6 mm I.D. |
|---|---|
| Eluent | 20 mM acetate buffer/methanol |
| Flow rate | 1.0 mL/min |
| Temperature | 30°C |
| Detection | UV at 220 nm |
Amitriptyline often exhibits peak tailing on competitive brands of columns but Pro C18 RS shows excellent peak shape for basic compounds.
| Column | 150 X 4.6 mm I.D. |
|---|---|
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (25/75) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
Pro C18 RS has excellent peak shape in a low-concentration TFA eluent that is suitable for LC/MS.
| Column | YMC-Pack Pro C18 RS (5 µm,
80 Å) 150 X 4.6 mm I.D. |
|---|---|
| Eluent | A) water/TFA (100/0.01) B) acetonitrile/TFA (100/0.01) 10-35%B (0-15min) |
| Flow rate | 0.2 mL/min |
| Temperature | 37°C |
| Detection | ESI positive-mode |
