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YMC-Pack Pro C18 RS

Pro C18 RS has excellent peak shape for the separation of basic compounds because of high quality end capping. Pro C18 RS has high recognition of stereoselectivity because of high carbon polymeric type bonding.
Pro C18 RS can be used over a wide pH range (1-10) to aid in method development.

SPECIFICATION

Particle size : 3µm, 5µm
Pore size : 80Å
Carbon contents : 22%
Usable pH range : 1.0-10.0

POINT

  • High selectivity,high carbon ODS column of polymeric type
  • Excellent separation of basic compounds
  • High quality end capping
  • Attached 2 types of inspection sheet
  • Low metal impurity silica base
  • High reproducibility: batch to batch and column to column
  • High durability
  • Choice of a wide range of eluent conditions,easy development of method.


High durability

pH 1

Pro C18 RS hardly change hydorophobicity of packing material in pH1 even 70ºC(column temperature)

pH 1

Change of hydorophobicity of material in 70ºC after the replacement of the solvent in the column with acetonitrile/water/TFA(10/90/1),

pH 10

You can use RS in alkaline eluent in which you can not use general ODS column

pH 10

Continuous analysis with 20mM H3BO3-NaOH(pH9.8)/methanol(50/50)

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A wide of choice of mobile phase facilitates method development

Separation behavior is influenced by changing the pH of the eluent. Pro C18 RS can operate over a wide pH range to allow manipulation of selectivity and retention.

pH3.2&pH6.8&pH8.9

  1. Flurbiprofen
  2. Methyl benzoate
  3. Lidocaine
  4. Diltiazem
  5. Verapamil
Column : YMC-Pack Pro C18 RS (5µm, 80Å)
150X4.6 mm I.D.
Eluent : 20mM acetate buffer/methanol
Flow rate : 1.0mL/min
Temperature : 30ºC
Detection : UV at 220 nm

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Separation of basic compounds

Amitriptyline often exhibits peak tailing on competitive brands of columns but Pro C18 RS shows excellent peak shape for basic compounds.

YMC-Pack Pro C18 RS&Brand D3

  1. Desipramine
  2. Diphenyl (I.S.)
  3. Imipramine
  4. Amitriptyline
Column size : 150X4.6 mm I.D.
Eluent : 20mM KH2PO4-K2HPO4
 (pH 6.9)/methanol (25/75)
Flow rate : 1.0mL/min
Temperature : 37ºC
Detection : UV at 254 nm

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Separation of peptides by LC/MS

Pro C18 RS has excellent peak shape in a low-concentration TFA eluent that is suitable for LC/MS.

Separation of peptides by LC/MS

  1. [Arg8]-Vasopressin
  2. Bradykinin
  3. Angiotensin II
  4. Oxytocin
  5. Angiotensin I
  6. Leu-Enkephalin
Column : YMC-Pack Pro C18 RS (5µm, 80Å)
150X4.6 mm I.D.
Eluent : A) water/TFA (100/0.01)
: B)acetonitrile/TFA (100/0.01)
10-35%B(0-15min)
Flow rate : 0.2mL/min
Temperature : 37ºC
Detection : ESI positve-mode

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