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YMC-Pack Polyamine II

YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.

SPECIFICATION

Particle size : 5µm
Pore size : 120Å
Usable pH range : 2.0-7.5

POINT

  • Silica gel chemically bonded to polyamine
  • The most suitable column for separation of sugars
  • Solves durability problem of conventional silica-based amino columns
  • Useful for separation of hydrophilic compounds including vitamins
  • Useful for separation of fat-soluble compounds using nonaqueous mobile phase


Excellent durability

Excellent durability

Flow conditions

Column : YMC-Pack Polyamine II 250 x 4.6mmI.D.
Eluent : acetonitrile/water
Flow Rate : 1.0mL/min
Temperature : ambient
Time : 100 hours

Analytical conditions

Eluent : acetonitrile/water(75/25)
Flow Rate : 1.0mL/min
Temperature : 26ºC
Detection : RI, 32 x 10-6 RIU/FS

Even in a hard flow durability test using mobile phase that consists only of water,the retention time of sugars changes little.

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The most suitable column for separation of sugars

The most suitable column for separation of sugars

  1. Fructose
  2. Glucose
  3. Sucrose
  4. Maltose
  5. Lactose
Column : YMC-Pack Polyamine II 250 x 4.6mmI.D.
Eluent : acetonitrile/water (75/25)
Flow Rate : 1.0mL/min
Temperature : 26ºC
Detection : RI, 32 x 10-6 RIU/FS

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For normal-phase separation

Tocopherol isomers

Column : YMC-Pack Polyamine II
 250 x 4.6mmI.D.
Eluent : n-hexane
 /ethyl acetate(70/30)
Flow Rate : 1.0mL/min
Temperature : 30ºC
Detection : UV at 295nm

L-ascorbic acid and erythobic acid

Column : YMC-Pack Polyamine II
 250 x 4.6mmI.D.
Eluent : 50mM NH4H2PO4
 /acetonitrile(30/70)
Flow Rate : 1.0mL/min
Temperature : 30ºC
Detection : UV at 250nm

YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.

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