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Flow reactor

YMC-Pack Ph

Unique Selectivity

Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.


Particle size : 3µm, 5µm
Pore size : 120Å
Carbon contents : 9%
Usable pH range : 2.0-7.5


  • Monomeric phenyl, fully endcapped
  • Preferential retention of aromatic compounds
  • Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules


Different selectivity between Ph and ODS

YMC-Pack Ph


  1. p-Aminobenzoic acid (PABA)
  2. Biotin (Vitamin H)
  3. Riboflavin (Vitamin B2)
  4. Cyanocobalamin (Vitamin B12)
Column : 150 x 4.6mmI.D.
Eluent : acetonitrile
 /50mM NH4H2PO4
Flow rate : 1.0 mL/min
Temperature : 37ºC
Detection : UV at 254 nm
Injection : 10µL (0.02-0.30mg/mL)

This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.

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