Bulk packings
BioPro IEX SmartSep Q/S and BioPro IEX Q/S, strong ion exchange media, are suitable for downstream chromatographic purification
processes in biopharmaceutical manufacturing. High dynamic binding capacity and high recovery of those media allow fast purification
processes at large scale. It offers high productivity on industrial purification.
BioPro IEX SmartSep Q/S, which show high resolution and recovery even at a high flow rate and high loading condition, are suitable
for an intermediate purification step and a polishing step of MAbs, proteins, peptides, and oligonucleotides.
BioPro IEX Q/S are designed for capture and intermediate purification of biopharmaceuticals such as MAbs.
| BioPro IEX SmartSep Q/S | BioPro IEX Q/S | |||
|---|---|---|---|---|
| BioPro IEX SmartSep Q | BioPro IEX SmartSep S | BioPro IEX Q | BioPro IEX S | |
| Matrix | Hydrophilic porous polymer | |||
| Particle size (µm) | 10, 20, 30 | 75 | ||
| Charged group | -R-N+(CH3)3 | -R-SO3- | -R-N+(CH3)3 | -R-SO3- |
| Ion-exchange capacity (meq/mL-resin) |
>0.08 | >0.10 | ||
| Dynamic binding capacity (DBC) (mg/mL-resin) |
>100 (BSA) | >100 (lysozyme) | >160 (BSA) | >160 (lysozyme) |
BioPro IEX SmartSep Q/S have higher DBC than conventional ion exchange media. In particular, for IgG, the DBC of BioPro IEX SmartSep Q/S is more than twice as high as that of the competitor.
This feature of BioPro IEX SmartSep Q/S makes purification productivity of IgG per unit time double or more.
| Conditions of DBC measurement * | |
| Column | 50 X 5.0 mmI.D. |
|---|---|
| Flow rate | 400 cm/hr (1.32 mL/min) |
| Temperature | 25°C |
*Please inquire us for deails.
| Particle size (µm) |
DBC (mg/mL-resin, 10% breakthrough) | |||
|---|---|---|---|---|
| Insulin | Lysozyme | Human Polyclonal lgG | ||
| BioPro IEX SmartSep S30 | 30 | 73 | 111 | 93 |
| Brand T (Porous S type) | 30 | 67 | 72 | 42 |
| Brand G (Porous S type) | 30 | 64 | 85 | 41 |
As the high DBC of BioPro IEX SmartSep Q/S is maintained at higher flow rates, it is suitable for high-speed purifications with 2-4 times the conventional flow rate. This feature offers a significant improvement of productivity.
| Column | 50 X 5.0 mmI.D. |
|---|---|
| Equilibration buffer | 20 mM citric acid-NaOH (pH 5.3) |
| Elution buffer | Equilibration buffer containing 0.5 M NaCl |
| Flow rate | 200-800 cm/hr (0.66-2.62 mL/min) |
| Temperature | ambient (25°C) |
| Detection | UV at 280 nm |
| Sample | 1.5 mg/mL human polyclonal IgG in equilibration buffer |
BioPro IEX SmartSep Q/S can be used at temperatures up to 60 °C, offering great flexibility in method development. The elution profile of an antisense oligonucleotide remained constant even after injecting the sample in 130 runs at 60 °C. There is no retention time shift and the column efficiency remains the same with BioPro IEX SmartSep Q.
| Column | BioPro IEX SmartSep Q20 100 X 4.6 mmI.D. |
|---|---|
| Eluent | A) 20 mM Tris-HCl (pH 8.6) B) 20 mM Tris-HCl (pH 8.6) containing 1 M NaCl |
| Gradient | 40-90%B (0-25 min), 90%B (25-35 min), 40%B (35.01-60 min) |
| Flow rate | 0.5 mL/min |
| Temperature | 60°C |
| Detection | UV at 260 nm |
| Injection | 12 µL |
| Sample | Firefly Luciferase GL2, antisense strand (5 nmol/mL) 5’-UCG AAG UAU UCC GCG UAC GdTdT-3 |
Cleaning in place (CIP) is an important procedure for cleaning and sterilizing columns used for protein purification. The DBC and the selectivity of proteins are unaffected following 20 cycles of CIP with 1 M NaOH. The high chemical stability of BioPro IEX Q/S allow effective cleaning with alkaline solution.
| Column | BioPro IEX S75, 50 X 5.0 mmI.D. |
|---|---|
| Linear velocity | 800 cm/hr |
| Equilibration buffer | 20 mM Glycine-NaOH (pH 9.0) |
| Elution buffer | 0.5 M NaCl in equilibration buffer |
| Sample | 1.0 mg/mL Lysozyme in equilibration buffer |
| Detection | UV at 300 nm |
