YMC-GPC is a column packed with highly cross-linked porous polystyrene/divinylbenzene media. It provides outstanding physical rigidity for extended lifetimes especially at a high temperatures and in aggressive solvents. YMC-GPC offers high productivity on preparative separation due to high resolution and high loadability, at a fast flow rate. Furthermore, higher resolution can be achieved on a sample that is hardly separated in combination with recycling chromatography method, even without changing mobile phase conditions or columns.
YMC-GPC has excellent solvent versatility. It can be transferred easily and rapidly between solvents of varying polarity. It is possible to select the optimum mobile phase depending on the solubility and separation behavior of the sample.
Recycling separation of polystyrene oligomers by YMC-GPC T4000 column is shown. By using recycling chromatography separation method, higher resolution can be achieved on a sample that is hardly separated, even without changing mobile phase conditions or columns. Furthermore, no solvent is consumed during recycling. It greatly contributes to reduction of solvent consumption on purification.
|Column|| YMC-GPC T4000 (10 μm, 100 Å)
600 X 21.2 mmI.D.
|Flow rate||10 mL/min|
|Detection||UV at 254 nm|
|Sample||polystyrene oligomers (50 mg/mL)|
Calibration curves of polystyrene by YMC-GPC are shown right. The calibration curves are designed to be linear over a specified molecular weight range, ensuring that the same degree of resolution is achieved across the full operating range of the column. Desired separation can be achieved by selecting a column depending on the molecular weight range of a sample.
|Column||300 X 7.5 mmI.D.|
|Flow rate||1.0 mL/min|