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Overview of method development for isolation/purification Analytical scale conditions Loading Scale-up Preparative separation conditions Effective preparative separation technique Examples of preparative purification

　　　

Strategy of method development for isolation/purification

Preparative HPLC is a separation on a greater scale using the same principles and method for analytical HPLC, but the differences are not just loading amount or column size. The purpose of preparative separation is to efficiently recover the compounds required. It is important to select preparative separation conditions from different aspects from analysis.

Steps for preparative purification

  1. Method development on the analytical scale

  2. Loading study

  3. Consideration for scaling up

  4. Method optimization on the preparative scale
  5. Performing preparative separation
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Columns

Bulk packings

Preparative LC systems