Biochromatography
YMC-Pack Diol-SEC
YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.
SPECIFICATION
| Particle size | : 5µm |
|---|---|
| Pore size | : 60Å, 120Å 200Å, 300Å |
| Usable pH range | : 5.0-7.5 |
POINT
- Utilizes silica gel base with high resistance to pressure
- Low-cost gel filtration column
- Useful for molecular weight determination of proteins and sugars
- Minimal secondary interactions
Calibration curves of proteins obtained using columns with different pore sizes
- Glutamate dehydrogenase
- Lactate dehydrogenase
- Enolase
- Adenylate kinase
- Cytochrome c
| Column | : YMC-Pack Diol 500 x 8.0 mmI.D. |
|---|---|
| Eluent | : 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl |
| Flow rate | : 0.7 mL/min |
| Temperature | : ambient |
| Detection | : UV at 280 nm |
Diol-120,Diol-200,and Diol-300 are suitable for the separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
For separation of proteins with molecular weights of 10,000 to several hundred thousands
- Glutamate dehydrogenase(MW 290,000)
- Lactate dehydrogenase(MW 142,000)
- Enolase(MW 67,000)
- Adenylate kinase(MW 32,000)
- Cytochrome c(MW 12,400)
| Column | : YMC-Pack Diol-SEC 500 x 8.0 mmI.D. |
|---|---|
| Eluent | : 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2M NaCl |
| Flow rate | : 0.7 mL/min |
| Temperature | : ambient |
| Detection | : UV at 280 nm |
For separation of peptides with molecular weights of 10,000 or less
- Insulin (Bovine)
- Neurotensin
- Angiotensin-II
- Glycine
| Column | : YMC-Pack Diol-60 500 x 8.0 mmI.D. |
|---|---|
| Eluent | : 0.1M KH2PO4-K2HPO4 (pH 7.0) containing 0.2MNaCl /acetonitrile (70/30) |
| Flow rate | : 0.7 mL/min |
| Temperature | : ambient |
| Detection | : UV at 215 nm |
| MW | |
| 1. Myoglobin 2. Ribonuclease A 3. Cytochrome c 4. Insulin (Bovine) 5. Insulin B chain 6. α-Mating factor 7. Neurotensin 8. Angiotensin-I 9. CCK-octapeptide 10. Bradykinin 11. Angiotensin-II 12. Oxytocin 13. Angiotensin-III 14. Met-enkephalin 15. Leu-enkephalin 16. Tetraglycine 17. Glycine |
17,000 13,700 12,400 5,700 3,496 1,684 1,672 1,296 1,143 1,060 1,040 1,007 931 573 555 246 75 |
For molecular weight determination of sugars
For separation or molecular weight determination of neutral sugars and water-soluble high-molecular-weight compounds,Diol-60,Diol-120,Diol-200,and Diol-300 are useful individually or in combination.