Columns

Size exclusion chromatography column
YMC-Pack Diol

YMC-Pack Diol-SEC is a size exclusion chromatography column utilizing a silica gel base. Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to several hundred thousands.
Diol-60 is the most suitable for separation of peptides of oligosaccharides whose molecular weights are 10,000 or less.

Lineup

Column Pore size (Å) Particle size (µm) Usable pH range Molecular weight range
Diol-60 60 3, 5 5.0-7.5 below 10,000
Diol-120 120 1,000-100,000
Diol-200 200 2, 3, 5 5,000-300,000
Diol-300 300 20,000-1,000,000
Points
  • Utilizes silica gel base with high resistance to pressure
  • Low-cost gel filtration column
  • Useful for molecular weight determination of proteins and sugars
  • Smooth method transfer from HPLC to UHPLC achieved by identical selectivity across particle sizes
  • Minimal secondary interactions

Application data