Bulk Packings
Preparative LC systems
Biopharmaceutical purification
Chiral technologies
Contract purification services
Flow reactor

Organic/inorganic hybrid silica packing materials

YMC-Triart and YMC-Triart Prep are bulk packing materials based on novel organic/inorganic hybrid silica particles. These materials can be widely applied from lab-scale purification to industrial production. Their excellent durability allows effective cleaning of the gel with alkaline solution. These materials provide excellent mechanical stability, and can be packed into a column repeatedly. The longer lifetime of YMC-Triart Prep greatly contributes to the reduction of production cost.



Product Particle size (µm) Pore size (Å) Carbon content (%) Usable pH range
Triart SIL 3, 5 120 - -
Triart C18 20 1.0-12.0
Triart C8 17
Triart Prep C18-S 10, 15, 20 120 20 2.0-10.0
Triart Prep C8-S 200 13
Triart Prep SIL 120, 200 - -

To YMC-Triart series


  • Bulk packing materials based on organic/inorganic hybrid
  • For lab-scale to production-scale purification
  • Excellent durability and long lifetime
  • Compatible with alkaline CIP

Versatile hybrid-based material

YMC-Triart is based on novel organic/inorganic hybrid particles. The particles combine the high mechanical stability and high efficiency of silica-based packing materials with the high chemical stability of polymer-based packing materials. A granulation process utilizing microreactor technology enables continuous and highly controlled production of the hybrid particles. The particles have uniform pore size distributions and smooth surfaces, as well as uniform particle sizes. This feature greatly contributes to excellent peak shape and separation reproducibility.

page top

Excellent mechanical stability

The YMC-Triart Prep material remains in its initial state after repacking more than 10 times. On the other hand, conventional silica shows pressure increases or crushed particles. The high mechanical stability of YMC-Triart Prep enables longer column lifetimes, and this feature reduces purification costs.

Column packing
Column size 100 X 50 mmI.D.
Packing pressure 6.5 MPa
Pressure measurement
Eluent methanol/water (85/15)
Flow rate 50 mL/min

page top

Easy scale-up from analytical to preparative

YMC-Triart Prep C18-S has identical selectivity to analytical Triart C18. A method established with analytical Triart C18 can be directly transferred to the preparative scale using the Triart Prep C18-S material.


1. Oxytocin (MW 1,007)
2. Met-Enkephalin (MW 574)
3. Leu-Enkephalin (MW 556)
4. Neurotensin (MW 1,673)
5. γ- Endorphin (MW 1,859)
6. β-Endorphin (MW 3,465)
Column : 150 X 4.6 mmI.D.
Eluent : A) water/TFA (100/0.1)
  B) acetonitrile/TFA (100/0.1)
  20-40%B (0-20 min)
Flow rate : 1.0 mL/min
Temperature : 37°C
Detection : UV at 220 nm
Injection : 10 µL (0.167 mg/mL)

page top

Excellent chemical durability

YMC-Triart Prep materials are highly acid-proof at lower pH conditions and alkaline-proof at higher pH conditions. These features enable purification with a mobile phase containing TFA and cleaning with alkaline solution, which are often used for peptide and protein purification.

Column 10 μm, 120 Å, 250 X 4.6 mmI.D.
Eluent acetonitrile/water (60/40)
Flow rate 1.0 mL/min
Temperature 37ºC
Detection UV at 254 nm
Sample n-butyl benzoate

Column 10 μm, 120 Å, 150 X 4.6 mmI.D.
Eluent 50 mM triethylamine in water/
50 mM triethylamine in methanol (80/20)
Flow rate 1.0 mL/min
Temperature 50ºC
Detection UV at 254 nm
Sample caffeine

page top

Regeneration with alkaline solution

After repeated injection of crude serum samples, the adsorption of proteins and/or other impurities on the surface of the packing material sometimes results in poor peak shape or degradation of retention capacity. In such cases, an alkaline washing procedure is generally adopted for regeneration and removal of impurities from the packing materials.
Hybrid silica-based YMC-Triart Prep, which shows strong resistance to high pH, allows effective cleaning of the gel with alkaline solution. This feature provides highly cost-effective purification of target compounds.


page top