Columns

Hydrophobic Interaction Chromatography Column
BioPro HIC HT/BioPro HIC BF

BioPro HIC columns are capable of separating proteins under non-denaturing conditions. BioPro HIC HT offers a time-critical analysis of biopharmaceuticals such as antibody-drug conjugates (ADCs). BioPro HIC BF is useful for separation of especially low hydrophobic proteins.

Specification

  Matrix Bonded
phase
Particle
size
(µm)
Usable
pH
range
Usable
temperature
range
Characteristics
BioPro HIC HT Hydrophilic
non-porous polymer
Butyl group 2.3 2-12 10-60°C First-choice HIC column for high resolution/high
throughput analyses
BioPro HIC BF 4 HIC column for separation of low hydrophobic
proteins

Comparison of retention behavior

BioPro HIC HT (first choice column) is suitable for successful separation of various biopharmaceuticals.
BioPro HIC BF shows the stronger retention of proteins than BioPro HIC HT due to the stationary phase hydrophobicity, and could be preferred for the separation of low hydrophobic proteins.

Column 100 X 4.6 mmI.D.
Eluent

A) 100 mM NaH2PO4-Na2HPO4 (pH 7.0) containing 2.0 M (NH4)2SO4
B) 100 mM NaH2PO4-Na2HPO4 (pH 7.0)
0%B (0-1 min), 0-100%B (1-11 min), 100%B (11-15 min)

Flow rate 0.5 mL/min
Temperature 25°C
Detection UV at 280 nm
Injection 15 µL
Sample

1. Adalimumab (0.5 mg/mL)
2. Trastuzumab (0.5 mg/mL)