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BioPro IEX Column

BioPro IEX Columns are for the separation of proteins, peptides, and nucleic acids. BioPro IEX Columns are available in QA and SP chemistries and are based on porous (QA and SP columns) and non-porous (QF and SF columns) hydrophilic polymer beads with low nonspecific adsorption. The porous materials offer excellent binding capacity with exceptionally high efficiency. The non-porous particles offer high efficiency and exceptional resolution.

To BioPro IEX series

Specification

  BioPro IEX QA BioPro IEX SP BioPro IEX QF BioPro IEX SF
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity (meq/mL-resin) 0.075-0.100 0.070-0.095 0.075-0.110 0.230-0.290
Dynamic binding capacity (mg/mL-resin) >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

POINTS

  • Newly developed hydrophilic polymer beads with low nonspecific adsorption
  • Effective surface structure designed to interact with biomolecules
  • High theoretical plate number and symmetrical peak shape by optimal packing technology
  • QA (quaternary ammonium) and SP (sulfopropyl) ion exchangers
  • Ideal for analysis and laboratory-scale purification on porous type with high binding capacity and high recovery of biomolecules
  • Ultra-fast analysis and high-resolution analysis on non-porous type


Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns

The separation of MAb is compared on SCX (BioPro IEX SF) and WCX (competitor’s) under the same gradient conditions at pH 5.6.
BioPro IEX SF column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.
Eluent : A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. : 35%B (70 mM NaCl)
Gradient slope : 0.25%B/min (0.5 mM NaCl)
Flow rate : 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D.,
0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature : 30°C
Detection : UV at 280 nm
Injection : 10 μL
Sample : Humanized monoclonal IgG1 (1 mg/mL)

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Excellent batch-to-batch reproducibility

BioPro IEX SF column exhibits excellent batch-to-batch reproducibility for MAb analysis, including the resolution of peaks for small charge variants. All the gel batches are inspected by various quality control tests, including HPLC analysis of MAb, and must pass rigorous criteria before release. BioPro IEX Columns are the best choice for the quality control of MAb and other biopharmaceuticals.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.
Column : BioPro IEX SF 5 μm, 100 X 4.6 mmI.D.
Eluent : A) 20 mM NaH2PO4-Na2HPO4 (pH 6.5)
B) 20 mM NaH2PO4-Na2HPO4 (pH 6.5) containing 0.2 M NaCl
0-50%B (0.5-30 min)
Flow rate : 0.5 mL/min (180 cm/hr)
Temperature : 25°C
Detection : UV at 215 nm
Injection : 10 μL
Sample : monoclonal antibody (IgG1)

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30 mm-length column for high-throughput analysis with low operating pressure

The separation performance of non-porous and porous columns for proteins is compared. BioPro IEX Colomn non-porous types are effective for high-throughput analysis at a high flow rate because of its high mechanical strength and high column efficiency.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.

  1. Ribonuclease A
  2. Cytochrome c
  3. Lysozyme
Eluent : A) 20 mM KH2PO4-K2HPO4 (pH 6.8)
B) 20 mM KH2PO4-K2HPO4 (pH 6.8) containing 0.5 M NaCl
0-100%B (0-4 min) for BioPro IEX SF
0-100%B (0-60 min) for BioPro IEX SP
Temperature : 25℃
Detection : UV at 220 nm
Injection : 20 µL

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High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, BioPro IEX QA has a superior DBC. Furthermore, its recovery is greater than that of conventional columns. The hydrophilic properties of the matrix polymer remarkably reduce the nonspecific adsorption of proteins on BioPro IEX columns.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

  Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)
Eluted amount
(mg/mL-resin)
Recovery*
(%)
BioPro IEX QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curves

Breakthrough curves
Column : BioPro IEX QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity : 3.0 cm/min
Sample : 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer : 20 mM Tris-HCl (pH 8.6)
Elution buffer : 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection : UV at 280 nm

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High loadability

BioPro IEX QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot achieve acceptable peak shapes and resolution even in small amount of injection.

Excellent peak shapes even at high sample load

YMC_BioPro QA 5µm,50x4.6 mml.D. Brand G(porous Q type) 10µm,50x5.0mml.D

  1. Ovalbumin
  2. Trypsin inhibitor
Eluent : A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1) containing 0.5 M NaCl
10-80%B (0-30 min)
Flow rate : 0.5 mL/min
Temperature : 25℃
Detection : UV at 280 nm
Injection : 100µL

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