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YMC-BioPro

YMC-BioPro ion exchange columns are for the separation of proteins, peptides, and nucleic acids. YMC-BioPro IEC columns are available in QA and SP chemistries and are based on porous (QA and SP columns) and non-porous (QA-F and SP-F columns) hydrophilic polymer beads with low nonspecific adsorption. The porous materials offer excellent binding capacity with exceptionally high efficiency. The non-porous particles offer high efficiency and exceptional resolution.

To BioPro series

Specification

  YMC-BioPro QA YMC-BioPro SP YMC-BioPro QA-F YMC-BioPro SP-F
Matrix hydrophilic porous polymer beads hydrophilic non-porous polymer beads
Particle size (µm) 5 3, 5
Charged group -CH2N+(CH3)3 -CH2CH2CH2SO3- -CH2N+(CH3)3 -CH2CH2CH2SO3-
Counter ion Cl- Na+ Cl- Na+
Ion-exchange capacity (meq/mL-resin) 0.075-0.100 0.070-0.095 0.075-0.110 0.230-0.290
Dynamic binding capacity (mg/mL-resin) >110 (BSA) >70 (human-IgG) >12 (BSA) >10 (human-IgG)
Usable temperature 4 - 60 ℃
Usable pH range 2-12
Column material PEEK

POINTS

  • Newly developed hydrophilic polymer beads with low nonspecific adsorption
  • Effective surface structure designed to interact with biomolecules
  • High theoretical plate number and symmetrical peak shape by optimal packing technology
  • QA (quaternary ammonium) and SP (sulfopropyl) ion exchangers
  • Ideal for analysis and laboratory-scale purification on porous type with high binding capacity and high recovery of biomolecules
  • Ultra-fast analysis and high-resolution analysis on non-porous type


Monoclonal antibody (MAb) analysis on non-porous type cation exchange columns

The separation of MAb is compared on SCX (YMC-BioPro SP-F) and WCX (competitor’s) under the same gradient conditions at pH 5.6.
The YMC-BioPro SP-F column provides higher resolution of MAb in a shorter analysis time than the competitor's column.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.
Eluent : A) 20 mM MES-NaOH (pH 5.6)
B) 20 mM MES-NaOH (pH 5.6) containing 0.2 M NaCl
Initial gradient conc. : 35%B (70 mM NaCl)
Gradient slope : 0.25%B/min (0.5 mM NaCl)
Flow rate : 180 cm/hr
(0.5 mL/min for 100 X 4.6 mmI.D., 0.378 mL/min for 250 X 4.0 mmI.D.)
Temperature : 30°C
Detection : UV at 280 nm
Injection : 10 μL
Sample : Humanized monoclonal IgG1 (1 mg/mL)

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Excellent batch-to-batch reproducibility

The YMC-BioPro SP-F column exhibits excellent batch-to-batch reproducibility for MAb analysis, including the resolution of peaks for small charge variants. All the gel batches are inspected by various quality control tests, including HPLC analysis of MAb, and must pass rigorous criteria before release. YMC-BioPro ion exchange columns are the best choice for the quality control of MAb and other biopharmaceuticals.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.
Column : YMC-BioPro SP-F 5 μm, 100 X 4.6 mmI.D.
Eluent : A) 20 mM NaH2PO4-Na2HPO4 (pH 6.5)
B) 20 mM NaH2PO4-Na2HPO4 (pH 6.5) containing 0.2 M NaCl
0-50%B (0.5-30 min)
Flow rate : 0.5 mL/min (180 cm/hr)
Temperature : 25°C
Detection : UV at 215 nm
Injection : 10 μL
Sample : monoclonal antibody (IgG1)

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30 mm-length column for high-throughput analysis with low operating pressure

The separation performance of non-porous and porous columns for proteins is compared. YMC-BioPro non-porous types are effective for high-throughput analysis at a high flow rate because of its high mechanical strength and high column efficiency.

Non-porous type YMC_BioPro SP_F 5µm,30x4.6 mml.D. Porous type YMC_BioPro SP 5µm,50x4.6 mml.D.

  1. Ribonuclease A
  2. Cytochrome c
  3. Lysozyme
Eluent : A) 20 mM KH2PO4-K2HPO4 (pH 6.8)
B) 20 mM KH2PO4-K2HPO4 (pH 6.8) containing 0.5 M NaCl
0-100%B (0-4 min) for YMC-BioPro SP-F
0-100%B (0-60 min) for YMC-BioPro SP
Temperature : 25℃
Detection : UV at 220 nm
Injection : 20µL

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High binding capacity and high recovery of biomolecules

Compared with conventional porous-polymer anion-exchange columns, YMC-BioPro QA has a superior DBC. Furthermore, its recovery is greater than that of conventional columns. The hydrophilic properties of the matrix polymer remarkably reduce the nonspecific adsorption of proteins on YMC-BioPro columns.

Comparisons in dynamic binding capacity (DBC) and recovery for BSA

  Dynamic binding capacity
(mg/mL-resin, 10% breakthrough)
Eluted amount
(mg/mL-resin)
Recovery*
(%)
YMC-BioPro QA 126 120 95
Brand T (porous Q type) 73 58 79
Brand G (porous Q type) 100 35 35

*Recovery: (Eluted amount/Dynamic binding capacity) X 100

Breakthrough curves

Breakthrough curves
Column : YMC-BioPro QA 50 X 4.6 mmI.D.
Brand T (porous Q Type) 50 X 4.6 mmI.D.
Brand G (porous Q Type) 50 X 5.0 mmI.D.
Linear velocity : 3.0 cm/min
Sample : 1 mg/mL Bovine serum albumin (BSA) in equilibration buffer
Equilibration buffer : 20 mM Tris-HCl (pH 8.6)
Elution buffer : 20 mM Tris-HCl (pH 8.6) containing 1.0 M NaCl
Detection : UV at 280 nm

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High loadability

YMC-BioPro QA shows the excellent peak shapes even when the loading amount increases. By contrast, the column of Brand G cannot achieve acceptable peak shapes and resolution even in small amount of injection.

Excellent peak shapes even at high sample load

YMC_BioPro QA 5µm,50x4.6 mml.D. Brand G(porous Q type) 10µm,50x5.0mml.D

  1. Ovalbumin
  2. Trypsin inhibitor
Eluent : A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1) containing 0.5 M NaCl
10-80%B (0-30 min)
Flow rate : 0.5 mL/min
Temperature : 25℃
Detection : UV at 280 nm
Injection : 100µL

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