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Silica based packing materials
YMC*GEL/YMC*GEL HG/YMC-Omega

YMC*GEL for preparative HPLC has various available functional groups, such as reversed-phase and normal-phase, and also various particle sizes from 3 μm with high-resolution to 150 μm for open column chromatography. YMC*GEL is able to work for a long period of time without particle breakage, as it uses high-density and high-strength silica gel. YMC*GEL/YMC*GEL HG packing materials are widely used both domestically and internationally.

POINTS

  • Packing materials with various functional groups and particle sizes are available
  • High density and high mechanical strength silica gel (YMC*GEL HG)
  • Suited for large-scale purification by using dynamic axial compression column
  • Packing materials for flash chromatography and open column chromatography are available

Excellent mechanical stability (YMC*GEL HG)

The high mechanical stability of YMC*GEL HG is demonstrated by means of repeated measurements with a dynamic axial compression column (DAC). Even after more than 10 repacking cycles with the same material, the pressure does not increase. The absence of fines is proven by a constant backpressure.

Packing conditions
Column size 100×50 mmI.D., Packing pressure : 6.5 MPa
Test conditions
Eluent methanol/water(85/15), Flow rate : 50 mL/min

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Suitable for separation of peptides

YMC*GEL/YMC*GEL HG are available with various functional groups and also with wide pores. Therefore, this material is suitable for the preparation of peptides, such as insulin and proteins. YMC*GEL HG can make scaling up from analytical scale to preparative scale easy, as it has the same performance as that of YMC-Pack.

Analytical condition (1), (3)
Column YMC-Pack C8
(5 μm, 200 Å)
150 X 4.6 mmI.D.
Eluent
A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 0.8 mL/min
Temperature ambient
Detection UV at 220 nm
Preparative condition (2)
Column :YMC*GEL C8
(10 μm, 200 Å)
150 X 50 mmI.D.
Eluent
A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 100 mL/min
Temperature ambient
Detection UV at 220 nm

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Purification of EPA-EE (YMC-Omega)

Optimization of the flow rate and sample loading for high yield was performed by using a preliminary column with 10 mmI.D., and the preparative column was scaled up by using dynamic axial compression columns with 200 mmI.D. Although using two times the linear flow rate slightly decreases the yield per preparation, the productivity can be increased because the preparation time per preparation is halved and the productivity per unit time increases 180%.

Column YMC-Omega (10 μm)
Eluent methanol
Temperature ambient
Detector UV at 210 nm
Sample 70% EPA-EE

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Lineup

YMC*GEL/YMC*GEL HG

Product Particle size (μm) Pore size (Å) Characteristics
ODS-A 3, 5 120, 200, 300
  • Standard ODS
ODS-A-HG 10, 15, 20, 50
ODS-AM 3, 5 120
  • outstanding lot-to-lot reproducibility
ODS-AQ 3, 5 120, 200
  • Superiour separation of hydrophilic compounds
ODS-AQ-HG 10, 15, 20, 50
C8 3, 5 120, 200, 300
  • Useful for separation of relatively highly hydrophobic compounds
  • Useful for separation of proteins and peptides
C8-HG 10, 15, 20, 50
C4 3, 5 120, 200, 300
  • Stationary phase with low hydrophobicity
  • Useful for separation of proteins and peptides
C4-HG 10, 15, 20, 50
TMS 3, 5 120
  • Reverse phased packing material with the lowest hydrophobicity
TMS-HG 10, 15, 20, 50
Ph(Phenyl) 3, 5 120
  • Different separation characteristics from C18
Ph-HG 10, 15, 20, 50
CN 3, 5 120
  • The medium polarity of the functional group allows selectable normal-phase and reversed-phase separation modes
CN-HG 10, 15, 20, 50
YMCbasic 3, 5, 10 -
  • Superior separation of basic compounds and peptides
SIL 3, 5 120, 200, 300
  • Standard of normal-phase packing material
SIL-HG 10, 15, 20, 50
Diol 5 120, 200, 300
  • Useful for gel filtration or normal-phase applications
Diol-HG 10, 15, 20, 50
NH2 5 120
  • Chemically bonded with aminopropyl groups
NH2-HG 10, 15, 20, 50

YMC-Omega

Product Particle size (μm) Pore size (Å) Characteristics
Omega 10, 20, 50 -
  • Superior separation of omega-fatty acids

Packing materials for Open column chromatography

Product Particle size (μm) Pore size (Å)
ODS-A 75, 150 120
SIL 75, 150 120

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