Columns

Hydrophobic Interaction Chromatography Column
BioPro HIC BF

Hydrophobic Interaction Chromatography Column, BioPro HIC BF, is effective for separation of proteins and antibodies such as Antibody-Drug Conjugates (ADCs).
BioPro HIC BF enables high resolution comparable to sub-3 µm with low operating pressure and fast analysis at high flow rates. It is also usable for laboratory-scale purification.

Specification
Matrix Hydrophilic non-porous polymer
Particle size 4 µm
Functional group Butyl group
Temperature range 10-60°C
pH range 2-12
Features

Effective for Drug-to-Antibody Ratio (DAR) analysis of ADCs

BioPro HIC BF column provides superior separation for ADCs and is effective for DAR determination.

Column BioPro HIC BF 4 µm, 100 X 4.6 mmI.D.
Eluent A) 50 mM NaH2PO4-Na2HPO4 (pH 7.0) containing
    1.5 M (NH4)2SO4/2-propanol (95/5)
B) 50 mM NaH2PO4-Na2HPO4 (pH 7.0)/2-propanol (80/20)
0%B (0-1 min), 0-100%B (1-15 min), 100%B (15-20 min)
Flow rate 1.0 mL/min
Temperature 25ºC
Injection volume 5 µm
Detection UV at 280 nm
Sample

Cysteine-conjugated ADC mimic  (5 mg/mL)

High resolution comparable to sub-3 µm with low operating pressure

Separation of proteins are compared between BioPro HIC BF with particle size 4 µm and a competitor’s HIC column with 2.5 µm. BioPro HIC BF with optimized media design and packing technology has superior resolution comparable to competitors’ columns with sub-3 µm.

BioPro HIC BF (4 µm)

Competitor HIC column (2.5 µm)

Column 100 X 4.6 mmI.D.
Eluent

A) 100 mM NaH2PO4-Na2HPO4 (pH 7.0) containing 2.0 M (NH4)2SO4
B) 100 mM NaH2PO4-Na2HPO4 (pH 7.0) 0-100%B (0-11 min), 100%B (11-15 min)

Flow rate 0.5 mL/min
Temperature 25ºC
Detection UV at 280 nm
Injection

15 µL

Sample

1. Myoglobin (0.73 mg/mL), 2. Ribonuclease A (0.75 mg/mL), 3. Lysozyme (0.25 mg/mL), 4. α-Chymotrypsinogen A (0.25 mg/mL)

Fast analysis at high flow rates

BioPro HIC BF is suitable for fast analysis at high flow rates because of its high mechanical stability of polymer beads and high column efficiency. It shows high resolution even at a high flow rate which competitor’s column has difficulty in using at.

Column 100 X 4.6 mmI.D.
Eluent

A) 100 mM NaH2PO4-Na2HPO4 (pH 7.0) containing 2.0 M (NH4)2SO4
B) 100 mM NaH2PO4-Na2HPO4 (pH 7.0)

Temperature 25ºC
Detection UV at 280 nm
Injection

15 µL

Sample

1. Myoglobin (0.73 mg/mL), 2. Ribonuclease A (0.75 mg/mL), 3. Lysozyme (0.25 mg/mL), 4. α-Chymotrypsinogen A (0.25 mg/mL)

Excellent peak shape under high loading

BioPro HIC BF column shows excellent peak shape when the loading amount increases. Therefore, it is useful for detection of minor peaks under overloading condition or for laboratory-scale purification.

Column BioPro HIC BF 4 µm, 100 X 4.6 mmI.D.
Eluent

A) 100 mM NaH2PO4-Na2HPO4 (pH 7.0)
     containing 2.0 M (NH4)2SO4
 B) 100 mM NaH2PO4-Na2HPO4 (pH 7.0)
60%B (0-0.5 min), 60-100%B (0.5-7.5 min), 100%B (7.5-10 min)

Flow rate 1.2 mL/min
Temperature 30ºC
Detection UV at 280 nm
Sample

Humanized monoclonal IgG (2.5 mg/mL)

Ordering information

Particle size
(µm)
Column size
inner diameter X length (mm)
Product number
4 4.6 X 100 BHB00S04-1046WT

Please contact us about the product with other sizes. Preparative columns are also available.