Biochromatography
Wide-Pore columns
YMC Co.,Ltd.'s Wide-Pore Columns are reversed-phase products available with 300Å or 200Å pore size.A choice of functional groups from ODS,C8,C4,and CN are also available, enabling the analyst to choose the proper pore size and stationary phase.
POINT
- Reversed-phase column with wide pore size
- Packing materials with pore size of 300Å or 200Å are available(For AQ,200Å only)
- Useful for separation of proteins or peptides
- Useful for fast analysis of compounds with high hydrophobicity
For compounds with high hydrophobicity
The chromatograms show a comparison of the retention behavior of alkylbenzenes on both ODS-A(300Å) and ODS-A(120Å). The compounds with relatively high hydropohbicity can be eluted in a shorter retention time on ODS-A(300Å) than ODS-A(120Å)
| Column | : YMC-Pack ODS-A (5µm, 120Å) and ODS-A (5µm, 300Å) 150 X 4.6mmI.D. |
|---|---|
| Eluent | : acetonitrile/water (75/25) |
| Flow rate | : 1.0mL/min |
| Temperature | : 30ºC |
| Detection | : UV at 254nm |
Selection of ODS-A(300Å),ODS-A(120Å) or C8 to optimize separation
Retention change per methylene group of ODS-A(300Å) is similar to that of ODS-A(120Å),indicating that alkyl chain density on the stationary phase is similar for these two columns. Since the slope of the line for ODS-A(300Å) is different from that for YMC-Pack C8,ODS-A(300Å) may show different separation behavior with regard to selectivity, despite having similar hydrophobicity. Thus, ODS-A(300Å),ODS-A(120Å) or C8 should be selected with care for rapid optimization of compound separation.
