Other Reversed Phase
YMC-Pack Ph
Unique Selectivity
Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
SPECIFICATION
| Particle size | : 3µm, 5µm 10µm |
|---|---|
| Pore size | : 120Å |
| Carbon contents | : 9% |
| Usable pH range | : 2.0-7.5 |
POINT
- Monomeric phenyl, fully endcapped
- Preferential retention of aromatic compounds
- Alternative to ODS or C4 bonded phases for the analysis or peptides and other biomolecules
APPLICATION DATA
Different selectivity between Ph and ODS
- p-Aminobenzoic acid (PABA)
- Biotin (Vitamin H)
- Riboflavin (Vitamin B2)
- Cyanocobalamin (Vitamin B12)
| Column | : 150 x 4.6mmI.D. |
|---|---|
| Eluent | : acetonitrile /50mM NH4H2PO4 (10/90) |
| Flow rate | : 1.0 mL/min |
| Temperature | : 37ºC |
| Detection | : UV at 254 nm |
| Injection | : 10µL (0.02-0.30mg/mL) |
This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.