Columns
Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120 Å and wide-pore 300 Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120 Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
| SPECIFICATION | |
| Particle size | 3 µm, 5 µm |
|---|---|
| Pore size | 120 Å |
| Carbon contents | 9% |
| Usable pH range | 2-7.5 |
This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.
| Column | 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/50 mM NH4H2PO4 (10/90) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
| Injection | 10 µL (0.02-0.30 mg/mL) |
