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YMC-Pack Diol-NP

A Normal Phase Silica Alternative

The YMC Diol-NP stationary phase is a versatile alternative to silica for normal phase separations. The bonded layer's-OH groups provide good selectivity without excessive retention, since hydrogen bonding with the Diol-NP layer is not as strong as one with the silanols on a bare silica surface.
Diol-NP columns also provide better reproducibility when compared with bare silica columns.

High Product Recovery

Like all YMC silica-based bonded phase,
the Diol-NP phase starts with an underlying silica support of exceptional purity.
YMC manufacturing and quality assurance possession ensure that the silica has a very low metal content. The silica purity greatly reduces nonspecific sample adsorption. As a result, you can expect excellent sample recovery

High Preparative Throughput

The high surface area of the underlying silica particle, together with the larger number of available sites for interaction of the 1, 2 dihydroxypropane ligands, results in good preparative loading.


Particle size : 5µm
Pore size : 60Å,120Å
Usable pH range : 2.0-7.5


  • Improved peak shape versus bare silica
  • Good selectivity without excessive retention
  • High recovery of product
  • High prep loading
  • Bonded phase reproducibility
  • Long column life

Separation feature of Diol-NP and SIL

YMC-Pack Diol-120-NP


  1. Phenol
  2. Catechol
  3. Resorcinol
  4. Hydroquinone
  5. Pyrogallol
  6. Phloroglucinol
Column size : 250 x 4.6mmI.D.
Eluent : hexane/ethanol (80/20)
Flow Rate : 1.0mL/min
Temperature : 30 ºC
Detection : UV at 254nm

This figure shows differences in selectivity of phenols when separated on Diol-NP and SIL under the same conditions. For these compounds, Diol columns provide longer retention time and better separation.

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