Columns
Bulk Packings
Preparative LC systems
Biopharmaceutical purification
Chiral technologies
Contract purification services
Flow reactor

J'sphere ODS-H80, M80, L80

High Efficiency

The 4µm, 80Å (80Å) silica support provides high chromatographic efficiency, while the very high surface area material (500 m2/g) offers exceptional interactions.This combination provides excellent peak symmetry.

Choose Your Retention

The J'sphere(TM) series combines three different carbon loads-9%, 14% and 22%-with a range of hydrogen-bonding capacities to provide three columns that differ in retention and often in selectivity as well.You can exploit these differences in method development without ever leaving the quality of product and support chromatographers have come to expect from YMC.

ODS-H80, with 22% carbon is the most hydrophobic and hydrolytically stable.It resists attack by both high- and low-pH solvents and has proven stable at pH's 1.1 to 9.3 for over 500 hours!

ODS-M80, with 14% carbon, is an inert,efficient phase with moderate hydrophobicity and hydrogen bonding capacity.

ODS-L80, with 9% carbon offers retention similar to C8, but with unique selectivity imparted by its high hydrogen-bonding capacity.

These three columns afford the chromatogapher a range of retention on a pure, high-efficiency, reproducible support.

SPECIFICATION

Particle size : 4µm
Pore size : 80Å
Carbon contents : ODS-H80 22%
: ODS-M80 14%
: ODS-L80 9%
Usable pH range : ODS-H80
 1.0-9.0
: ODS-M80
: ODS-L80
 2.0-7.5

POINT

  • Average over 100,000 plates / meter
  • Inert, reproducible support
  • Choice of three levels of hydrophobicity
  • Excellent peak symmetry with basic molecules


Comparison of hydrophobicity & hydrogen bonding capacity with competitive ODS

Comparison of hydrophobicity with competitive ODS

Column : 150 X 4.6mmI..D.
Eluent : acetonitrile
 /water(75/25)
Flow rate : 1.0mL/min
Temperature : 37ºC
Detection : UV at 254nm

Comparison of hydrogen bonding capacity with competitive ODS

Column : 150 X 4.6mmI..D.
Eluent : acetonitrile
 /20mM KH2PO4(15/85)
Flow rate : 1.0mL/min
Temperature : 37ºC
Detection : UV at 254nm

Three different types of ODS with different ligand coverage display different hydrophobicity and hydrogen bonding capacities and steric selectivity. ODS-H80 has higher hydrophobicity and less hydrogen bonding capacity. ODS-M80 has moderate hydrophobicity and hydrogen bonding capacity. ODS-L80 has less hydrophobicity similar to a C8 and high hydrogen-bonding capacity.

page top

Under acid/alkaline conditions

Under acidic conditions

Column : J'sphere ODS-H80
 150 x 4.6 mmI.D.
Eluent : acetonitrile
 / water(60/40)
Flow rate : 1.0mL/min
Temperature : 37ºC
Detection : UV at 270nm
Sample : butyl benzoate

Under alkaline conditions

Column : J'sphere ODS-H80
 150 x 4.6 mmI.D.
Eluent : acetonitrile
 / water(60/40)
Flow rate : 1.0mL/min
Temperature : 37ºC
Detection : UV at 270nm
Sample : butyl benzoate

ODS-H80 shows acid resistance and alkali resistance. It is effective for separation of peptides and basic compounds.

page top