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YMC-Triart Diol-HILIC

YMC-Triart Diol-HILIC is a HILIC (hydrophilic interaction chromatography) column based on an organic/inorganic hybrid particle synthesized with dihydroxypropyl groups. Triart Diol-HILIC is ideal for a separation of polar and hydrophilic compounds that are not retained by reversed-phase (C18, C8, and others) chromatography. Triart Diol-HILIC begins with an organic/inorganic hybrid-based particle with excellent durability and usability across a wide pH range. Excellent reproducibility is achieved by the use of the dihydroxypropyl group–a neutral functional group exhibiting low nonspecific adsorption.

SPECIFICATION

Base : organic/inorganic hybrid silica
Particle size : 1.9 μm, 3 μm, 5 μm
Pore size : 120Å
Usable pH range : 2-10

POINT

  • HILIC column based on an organic/inorganic hybrid silica
  • Useful for a separation of polar and hydrophilic compounds
  • Highly-improved chemical durability in high pH conditions
  • Excellent versatility : wide pH range of application
  • Excellent reproducibility : low nonspecific adsorption

Useful for separation of polar and hydrophilic compounds

Triart C18 (reversed-phase) shows very weak retention and poor resolution of L-ascorbic acid and its stereoisomer (erythorbic acid), even with a 100% aqueous mobile phase. However, Triart Diol-HILIC shows strong retention and better resolution of these compounds with a mobile phase containing 90% organic solvent.

分離モードの変更

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Column 5 μm, 150 X 3.0 mmI.D.
Flow rate 0.425 mL/min
Temperature 40°C
Detection UV at 254 nm
Injection 4  μL (0.05 mg/mL)

Excellent durability and reproducibility at high pH and elevated temperatures

Triart Diol-HILIC provides highly reproducible separations, even at high pH and high temperatures. The lifetime of Triart Diol-HILIC is much greater than that of conventional silica-based diol columns.

Durability in high pH (pH 11.5, 50ºC)

ss

Column 5 μm, 150 X 4.6 mmI.D.
Eluent acetonitrile/water/NH3 (90/10/0.1) pH 11.3
Temperature 50°C
Flow rate 1.0 mL/min
Sample Cytosine

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