Columns

Size exclusion column for monoclonal antibody (MAb) separation
YMC-SEC MAB

YMC-SEC MAB is a silica-based size exclusion chromatography (SEC)/gel filtration chromatography (GFC) column with excellent durability and batch-to-batch reproducibility. Optimized base material design and surface modification provide superior separation of antibody monomers and their aggregates/fragments with very low nonspecific adsorption. This is ideal for the quality control assessment of antibody drugs.

Specification
Base Silica gel
Particle size 3 µm
Pore size 250 Å
Bonded phase

Dihydroxypropyl

Usable pH range 5-7.5
Molecular weight range

10,000-700,000

Features

Suitable for separation of aggregates of Antibodies or Antibody Drug Conjugates (ADCs)

A humanized monoclonal antibody and an ADC were analyzed using YMC-SEC MAB. Chromatograms show excellent peak shape, and good separation between aggregates and a monomer peak. YMC-SEC MAB is effective for characterization and quality control of antibody drugs.

Analysis of a humanized monoclonal antibody

Column YMC-SEC MAB (3 µm, 250 Å)

300 X 4.6 mmI.D.

Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0)
containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25ºC
Detection UV at 280 nm
Injection 10 µL
Sample

Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column YMC-SEC MAB (3 µm, 250 Å)

300 X 4.6 mmI.D.

Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0)
containing 0.2 M NaCl/2-propanol (85/15)
Flow rate 0.165 mL/min
Temperature 25ºC
Detection UV at 280 nm
Injection 4 µL
Sample

Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

YMC-SEC MAB has excellent repeatability of separation without any change of theoretical plate number and elution time even after repeated separation of a monoclonal antibody for more than 500 times because of its superior durability.

Column YMC-SEC MAB (3 µm, 250 Å)

300 X 4.6 mmI.D.

Eluent 0.1 M NaH2PO4-Na2HPO4 (pH 7.0)
containing 0.2 M NaCl
Flow rate 0.33 mL/min
Temperature 25ºC
Detection UV at 280 nm
Injection 2 µL
Sample

Humanized monoclonal antibody (1 mg/mL)

Superior batch-to-batch reproducibility

YMC-SEC MAB provides excellent reproducibility of the separation of monomer and aggregates as well as for monomer and fragments. Therefore, it is very effective for quality control of antibody drugs.

Column YMC-SEC MAB (3 µm, 250 Å)

300 X 4.6 mmI.D.

Eluent 0.1 M KH2PO4-K2HPO4 (pH 7.0)
containing 0.2 M NaCl
Flow rate 0.165 mL/min
Temperature 25ºC
Detection UV at 280 nm
Injection 10 µL
Sample

Humanized monoclonal antibody (5 mg/mL)

Ordering Information

Particle size
(µm)
Column size
inner diameter X length (mm)
Product number
3 4.6 X 150 DLM25S03-1546WT
4.6 X 300 DLM25S03-3046WT
8.0 X 300 DLM25S03-3008WT

Please inquire us for other than listed above.