Size exclusion column for monoclonal antibody (MAb) separation
YMC-SEC MAB

• Product Information
• Care and Use Instructions

• Catalog Download
• Bioseparation catalog

• Ordering Information

Features

• Suitable for separation of aggregates of Antibodies or Antibody Drug Conjugates (ADCs)
• Excellent durability
• Superior batch-to-batch reproducibility

Suitable for separation of aggregates of Antibodies or Antibody Drug Conjugates (ADCs)

A humanized monoclonal antibody and an ADC were analyzed using YMC-SEC MAB. Chromatograms show excellent peak shape, and good separation between aggregates and a monomer peak. YMC-SEC MAB is effective for characterization and quality control of antibody drugs.

Analysis of a humanized monoclonal antibody

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Analysis of an antibody-drug conjugate (ADC)

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15)
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	4 µL
Sample	Cystein-conjugated ADC mimic (2.5 mg/mL)

Excellent durability

YMC-SEC MAB has excellent repeatability of separation without any change of theoretical plate number and elution time even after repeated separation of a monoclonal antibody for more than 500 times because of its superior durability.

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M NaH2PO4-Na2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate	0.33 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	2 µL
Sample	Humanized monoclonal antibody (1 mg/mL)

Superior batch-to-batch reproducibility

YMC-SEC MAB provides excellent reproducibility of the separation of monomer and aggregates as well as for monomer and fragments. Therefore, it is very effective for quality control of antibody drugs.

Column	YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D.
Eluent	0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl
Flow rate	0.165 mL/min
Temperature	25°C
Detection	UV at 280 nm
Injection	10 µL
Sample	Humanized monoclonal antibody (5 mg/mL)

Ordering Information

Please inquire us for other than listed above.

Technical Information

• Tips for optimization of peptides and proteins separation by reversed-phase