Pro C18 RS has excellent peak shape for the separation of basic compounds because of high quality end capping. Pro C18 RS has high recognition of stereoselectivity because of high carbon polymeric type bonding.
Pro C18 RS can be used over a wide pH range (1-10) to aid in method development.
SPECIFICATION
| Particle size | : 3µm, 5µm |
|---|---|
| Pore size | : 80Å |
| Carbon contents | : 22% |
| Usable pH range | : 1.0-10.0 |
POINT
- High selectivity,high carbon ODS column of polymeric type
- Excellent separation of basic compounds
- High quality end capping
- Attached 2 types of inspection sheet
- Low metal impurity silica base
- High reproducibility: batch to batch and column to column
- High durability
- Choice of a wide range of eluent conditions,easy development of method.
High durability
pH 1
Pro C18 RS hardly change hydorophobicity of packing material in pH1 even 70ºC(column temperature)
Change of hydorophobicity of material in 70ºC after the replacement of the solvent in the column with acetonitrile/water/TFA(10/90/1),
pH 10
You can use RS in alkaline eluent in which you can not use general ODS column
Continuous analysis with 20mM H3BO3-NaOH(pH9.8)/methanol(50/50)
A wide of choice of mobile phase facilitates method development
Separation behavior is influenced by changing the pH of the eluent. Pro C18 RS can operate over a wide pH range to allow manipulation of selectivity and retention.
- Flurbiprofen
- Methyl benzoate
- Lidocaine
- Diltiazem
- Verapamil
| Column | : YMC-Pack Pro C18 RS (5µm, 8µm) 150X4.6 mm I.D. |
|---|---|
| Eluent | : 20mM acetate buffer/methanol |
| Flow rate | : 1.0mL/min |
| Temperature | : 30ºC |
| Detection | : UV at 220 nm |
Separation of basic compounds
Amitriptyline often exhibits peak tailing on competitive brands of columns but Pro C18 RS shows excellent peak shape for basic compounds.
- Desipramine
- Diphenyl (I.S.)
- Imipramine
- Amitriptyline
| Column size | : 150X4.6 mm I.D. |
|---|---|
| Eluent | : 20mM KH2PO4-K2HPO4 (pH 6.9)/methanol (25/75) |
| Flow rate | : 1.0mL/min |
| Temperature | : 37ºC |
| Detection | : UV at 254 nm |
Separation of peptides by LC/MS
Pro C18 RS has excellent peak shape in a low-concentration TFA eluent that is suitable for LC/MS.
- [Arg8]-Vasopressin
- Bradykinin
- Angiotensin II
- Oxytocin
- Angiotensin I
- Leu-Enkephalin
| Column | : YMC-Pack Pro C18 RS (5µm, 8µm) 150X4.6 mm I.D. |
|---|---|
| Eluent | : A) water/TFA (100/0.01) : B)acetonitrile/TFA (100/0.01) 10-35%B(0-15min) |
| Flow rate | : 0.2mL/min |
| Temperature | : 37ºC |
| Detection | : ESI positve-mode |