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YMC-UltraHT Pro C18 / YMC-Pack Pro C18

The choice of columns for analysis of difficult compounds by HPLC YMC-Pack Pro C18 columns start with a tightly controlled, highly purified silica gel, followed by a high-coverage C18 bonding and finish with a unique endcapping procedure utilizing Lewis acid-Lewis base chemistry. 2 μm particle size columns YMC-UltraHT series are specifically designed for high-speed and high throughput analysis.
Together, these provide unequaled separations with sharp and symmetrical peaks for all types of organic molecules, especially basic pharmaceuticals and chelating compounds.

SPECIFICATION

Particle size :2 µm,3 µm,5 µm,10 µm
Pore size :120 Å
Carbon contents :16%
Usable pH range :2.0-8.0

POINTS

  • High performance, reproducible, reliable columns designed for the most demanded HPLC
  • Made with highly inert, ultrapure, pH neutral silica
  • Extremely rigorous physical and chemical specifications
  • Novel Lewis acid/Lewis base endcapping
  • Very high efficiency columns, typically 100,000 to 120,000 plates/metre for 5µm and 130,000 to 150,000 plates/metre for 3µm packings
  • Continues YMC proven batch-to-batch and column-to-column reproducibility


Effective for separation of basic compounds

Effective for separation of basic compounds

Tricyclic antidepressants

  1. Desipramine hydrochloride
  2. Acenaphthene(I.S.)
  3. Imipramine hydrochloride
  4. Amitriptyline hydrochloride
Column : YMC-Pack Pro C18 (5 µm,120 Å) 150X4.6 mmI.D.
Eluent : 20 mM KH2PO4-K2HPO4 (pH 6.9) / methanol (25/75)
Flow rate : 1.0 mL/min
Temperature : 37ºC
Detection : UV at 254 nm

Basic peptides

Basic peptides

  1. Bradykinin
  2. Dynorphin A (Human,1-13)
  3. Eledoisin related peptide
  4. Neurotensin
  5. Bombesin
  6. Substance P

Neutral peptides

Neutral peptides

  1. Oxytocin
  2. Met-Enkephalin
  3. Leu-Enkephalin
  4. Angiotensin I
  5. α-Mating factor
  6. Insulin
Column : YMC-Pack Pro C18 and ODS-A (5 µm,120 Å) 75 X 4.6 mmI.D.
Eluent : A) water/TFA (100/0.1)      B) acetonitrile/TFA (100/0.1)
  20-40%B (0-20 min, linear)
Flow rate : 1.0 mL/min
Temperature : 37 ºC
Detection : UV at 220 nm

YMC-Pack Pro C18 is manufactured with a unique endcapping procedure and provides excellent peak symmetry for Basic peptides and equal separation performance for neutral peptides compared with ODS-A.

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Excellent reproducibility

batch-to-batch Reproducibility of the above three lots

Excellent reproducibility for separation of hydrophobic, hydrophilic, basic, and acidic compounds.

column-to-column

YMC controls packing condition under stringent quality control criteria. That is because YMC provides columns of high theoretical plate number and consistently reproducible quality.

Batch certificates

Batch certificates

Batch certificates show basic properties of raw material silica gel such as particle size, pore size and metal content.
Carbon load after bonding and results of a separation with standard test mixture are shown.

Column inspection reports

Column inspection reports

Columns inspection reports that show column quality such as theoretical plate number and asymmetry factor are also shown. All analysis conditions including the sample concentration are shown so the analyst may periodically use the same conditions to evaluate column degradation.

Attached batch certificates and column inspection reports prove batch-to-batch and column-to- column reproducibility.

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2 µm columns applicable for conventional LC system

2 μm perticle size columns included in Pro C18 can be used for ultra fast analysis with conventional LC, as its pressure is lower than commercial sub-2 μm columns. Considering the extra column band broadening, the column of 3.0 mmI.D. is more applicable for conventional LC systems than the column of 2.0 mmI.D..

50 X 3.0 mmI.D. / 50 X 2.0 mmI.D.

Cephalosporin antibiotics

  1. Cephalexin
  2. Cefaclor
  3. Cephaloglycin
  4. Cephaloridine
Column : YMC-UltraHT Pro C18
Eluent : acetonitrile/20 mM KH2PO4 (10/90)
Temperature : 37ºC
Detection : UV at 260 nm

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