Normal Phase
YMC-Pack Polyamine II
YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.
SPECIFICATION
| Particle size | : 5µm |
|---|---|
| Pore size | : 120Å |
| Usable pH range | : 2.0-7.5 |
POINT
- Silica gel chemically bonded to polyamine
- The most suitable column for separation of sugars
- Solves durability problem of conventional silica-based amino columns
- Useful for separation of hydrophilic compounds including vitamins
- Useful for separation of fat-soluble compounds using nonaqueous mobile phase
Trouble shooting tips
Excellent durability
Flow conditions
| Column | : YMC-Pack Polyamine II 250 x 4.6mmI.D. |
|---|---|
| Eluent | : acetonitrile/water |
| Flow Rate | : 1.0mL/min |
| Temperature | : ambient |
| Time | : 100 hours |
Analytical conditions
| Eluent | : acetonitrile/water(75/25) |
|---|---|
| Flow Rate | : 1.0mL/min |
| Temperature | : 26ºC |
| Detection | : RI, 32 x 10-6 RIU/FS |
Even in a hard flow durability test using mobile phase that consists only of water,the retention time of sugars changes little.
The most suitable column for separation of sugars
- Fructose
- Glucose
- Sucrose
- Maltose
- Lactose
| Column | : YMC-Pack Polyamine II 250 x 4.6mmI.D. |
|---|---|
| Eluent | : acetonitrile/water (75/25) |
| Flow Rate | : 1.0mL/min |
| Temperature | : 26ºC |
| Detection | : RI, 32 x 10-6 RIU/FS |
For normal-phase separation
| Column | : YMC-Pack Polyamine II 250 x 4.6mmI.D. |
|---|---|
| Eluent | : n-hexane /ethyl acetate(70/30) |
| Flow Rate | : 1.0mL/min |
| Temperature | : 30ºC |
| Detection | : UV at 295nm |
| Column | : YMC-Pack Polyamine II 250 x 4.6mmI.D. |
|---|---|
| Eluent | : 50mM NH4H2PO4 /acetonitrile(30/70) |
| Flow Rate | : 1.0mL/min |
| Temperature | : 30ºC |
| Detection | : UV at 250nm |
YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.