YMC-Pack PROTEIN-RP is a reversed-phase column utilizing a silica gel base. It contains a stationary phase, specifically designed for separation of proteins or peptides.
Problems that are associated with conventional reversed-phase columns with short alkyl chain lengths are minimized.Robust column lifetime and excellent recovery of hydrophobic proteins are typically possible on this phase.
Particle size | : 5µm |
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Pore size | : 200Å |
Usable pH range | : 1.5-7.5 |
Sample | Recovery (%) | ||
---|---|---|---|
YMC-Pack PROTEIN-RP |
C4 column, Company A |
C4 column, Company B |
|
Ribonuclease A | 93 | 95 | 86 |
Cytochrome c | 94 | 89 | 95 |
Lysozyme | 98 | 93 | 76 |
Myoglobin | 97 | 85 | 93 |
BSA | 94 | 92 | 86 |
Ovalbumin | 90 | 73 | 88 |
Transferrin | 94 | 98 | 88 |
Insulin(bovine) | 97 | 73 | 92 |
Insulin B chain | 82 | 76 | 70 |
α-Mating factor | 93 | 82 | 81 |
Leu-Enkephalin | 92 | 84 | 91 |
Gly-Gly-Gly-Gly | 95 | 86 | 70 |
Recovery of various proteins and peptides is shown.
YMC-Pack PROTEIN-RP gives greater recovery than competitive C4 columns.
Flow conditions
Eluent | : water/TFA (100/0.1) |
---|---|
Flow rate | : 1.0 mL/min |
Temperature | : ambient |
Measurement conditions
Column | : YMC-Pack PROTEIN-RP 250 x 4.6 mmI.D. |
---|---|
Eluent | : acetonitrile / water (40/60) |
Flow rate | : 1.0 mL/min |
Temperature | : 30ºC |
Detection | : UV at 254 nm, 0.32AUFS |
The graph shows the test result of the stability of the stationary phase with 0.1% aqueous TFA. Retention of diphenyl on C4 columns manufactured by other companies greatly decreases as time passes. This is caused by detachment of butyl groups from the packing material due to acid hydrolysis.
Retention of diphenyl on YMC-Pack PROTEIN-RP is shown to be stable after 500 hours of mobile phase flow.