Columns

Alcyon SFC

Alcyon SFC columns are Supercritical Fluid Chromatography (SFC) columns available with chiral/achiral stationary phases. Alcyon SFC columns are specifically packed in SFC-compatible hardware and are tested under SFC conditions.
The low viscosity of supercritical carbon dioxide allows for analytical separations that are 3–5 times faster than those for normal-phase HPLC.

POINT
  • Available in chiral and achiral stationary phases
  • Faster separation with high resolution
  • Excellent durability
  • Great reduction of solvent consumption
Features

Chiral and achiral stationary phases

Chiral Immobilized type

  Particle size
(µm)
Chiral selector Column size*
inner diameter X length (mm)
Amylose-SA 3
5
2.1 X 150
3.0 X 50
3.0 X 100
3.0 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
Cellulose-SB
Cellulose-SC
Cellulose-SJ
Cellulose-SZ

* See Product List for details of column size lineup.

Chiral Coated type

  Particle size
(µm)
Chiral selector Column size*
inner diameter X length (mm)
Amylose-C        
Amylose-C Neo
3
5
2.1 X 150
3.0 X 50
3.0 X 100
3.0 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
Cellulose-C

* See Product List for details of column size lineup.

Achiral

  Particle size
(µm)
Functional group サイズラインナップ
内径 X 長さ (mm)
Triart C18 5 -C18H37 2.1 X 150
4.6 X 150
4.6 X 250
Triart Diol
Triart PFP
CN -(CH2)3CN 2.1 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
SIL (-OH)

Faster separation with high resolution

Faster chiral separation of trans-Stilbene oxide is achieved using supercritical fluid chromatography compared with HPLC separation. The lower viscosity and larger diffusion coefficient of the supercritical fluid provide rapid separation of both chiral and achiral compounds.

SFC
Column Alcyon SFC CSP Amylose-C Neo
5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (80/20)
Flow rate 3.0 mL/min
Temperature 40°C
Detection UV at 220 nm
Back pressure 13.8 MPa (2000 psi)
HPLC
Column CHIRAL ART Amylose-C Neo
5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (90/10)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm

Excellent peak shape ~Good peak shape in mobile phase without the addition of acid~

Excellent peak shape of 2-Phenylpropionic acid is obtained using SFC chiral separation. Under HPLC conditions, the peak shape is very broad with mobile phases containing no additives, such as acid. However, using SFC, the peak shapes are very good with just a mixture of CO2 and methanol. Supercritical carbon dioxide is considered to act as an weak acid.

SFC
Column Alcyon SFC CSP Cellulose-C
5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (98/2)
Flow rate 3.0 mL/min
Temperature 35°C
Detection UV at 220 nm
Back pressure 13.8 MPa (2000 psi)
HPLC
Column CHIRAL ART Cellulose-C
5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (99/1)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 220 nm

Selectable column to suit the sample characteristics

The results of analyzing phenols on three kinds of achiral columns are shown. As the separation behavior is different, a column can be selected to suit the sample characteristics.
YMC offers five achiral-phase SFC columns.

Column Alcyon SFC Triart Diol
5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (88/12)
Flow rate 3.0 mL/min
Temperature 30°C
Detection UV at 230 nm
Back pressure 13.8 MPa (2000 psi)

SFC preparative separation of flavanone

Alcyon SFC columns show excellent peak shape, even for faster separations. As a result, high-purity and high-recovery purification will be achieved. Alcyon SFC columns offer purification with higher efficiency owing to lower solvent consumption.

  Fr.1 Fr.2
Enantiomeric purity 99.9%ee 99.7%ee
Yield 95.7% >99.9%
Column Alcyon SFC CSP Amylose-C Neo
5 µm, 250 X 20 mmI.D.
Eluent CO2/ethanol (80/20)
Flow rate 60 mL/min
Temperature 30°C
Detection UV at 280 nm
Back pressure 15 MPa (2180 psi)
Injection 3 mL (20 mg/mL)
Injected every 2.65 minutes