Alcyon SFC columns are Supercritical Fluid Chromatography (SFC) columns available with chiral/achiral stationary phases. Alcyon SFC columns are specifically packed in SFC-compatible hardware and are tested under SFC conditions.
The low viscosity of supercritical carbon dioxide allows for analytical separations that are 3–5 times faster than those for normal-phase HPLC.
Particle size (µm) |
Chiral selector | Column size* inner diameter X length (mm) |
||
---|---|---|---|---|
Amylose-SA | 3 5 |
2.1 X 150 3.0 X 50 3.0 X 100 3.0 X 150 4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
||
Cellulose-SB | ||||
Cellulose-SC | ||||
Cellulose-SJ | ||||
Cellulose-SZ |
* See Product List for details of column size lineup.
Particle size (µm) |
Chiral selector | Column size* inner diameter X length (mm) |
||
---|---|---|---|---|
Amylose-C Amylose-C Neo |
3 5 |
2.1 X 150 3.0 X 50 3.0 X 100 3.0 X 150 4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
||
Cellulose-C |
* See Product List for details of column size lineup.
Particle size (µm) |
Functional group | サイズラインナップ 内径 X 長さ (mm) |
|
---|---|---|---|
Triart C18 | 5 | -C18H37 | 2.1 X 150 4.6 X 150 4.6 X 250 |
Triart Diol | |||
Triart PFP | |||
CN | -(CH2)3CN | 2.1 X 150 4.6 X 150 4.6 X 250 10 X 250 20 X 250 |
|
SIL | (-OH) |
Faster chiral separation of trans-Stilbene oxide is achieved using supercritical fluid chromatography compared with HPLC separation. The lower viscosity and larger diffusion coefficient of the supercritical fluid provide rapid separation of both chiral and achiral compounds.
SFC | |
Column | Alcyon SFC CSP Amylose-C Neo 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | CO2/methanol (80/20) |
Flow rate | 3.0 mL/min |
Temperature | 40°C |
Detection | UV at 220 nm |
Back pressure | 13.8 MPa (2000 psi) |
HPLC | |
Column | CHIRAL ART Amylose-C Neo 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | n-hexane/2-propanol (90/10) |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Detection | UV at 230 nm |
Excellent peak shape of 2-Phenylpropionic acid is obtained using SFC chiral separation. Under HPLC conditions, the peak shape is very broad with mobile phases containing no additives, such as acid. However, using SFC, the peak shapes are very good with just a mixture of CO2 and methanol. Supercritical carbon dioxide is considered to act as an weak acid.
SFC | |
Column | Alcyon SFC CSP Cellulose-C 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | CO2/methanol (98/2) |
Flow rate | 3.0 mL/min |
Temperature | 35°C |
Detection | UV at 220 nm |
Back pressure | 13.8 MPa (2000 psi) |
HPLC | |
Column | CHIRAL ART Cellulose-C 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | n-hexane/2-propanol (99/1) |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Detection | UV at 220 nm |
The results of analyzing phenols on three kinds of achiral columns are shown. As the separation behavior is different, a column can be selected to suit the sample characteristics.
YMC offers five achiral-phase SFC columns.
Column | Alcyon SFC Triart Diol 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | CO2/methanol (88/12) |
Flow rate | 3.0 mL/min |
Temperature | 30°C |
Detection | UV at 230 nm |
Back pressure | 13.8 MPa (2000 psi) |
Alcyon SFC columns show excellent peak shape, even for faster separations. As a result, high-purity and high-recovery purification will be achieved. Alcyon SFC columns offer purification with higher efficiency owing to lower solvent consumption.
Fr.1 | Fr.2 | |
---|---|---|
Enantiomeric purity | 99.9%ee | 99.7%ee |
Yield | 95.7% | >99.9% |
Column | Alcyon SFC CSP Amylose-C Neo 5 µm, 250 X 20 mmI.D. |
---|---|
Eluent | CO2/ethanol (80/20) |
Flow rate | 60 mL/min |
Temperature | 30°C |
Detection | UV at 280 nm |
Back pressure | 15 MPa (2180 psi) |
Injection | 3 mL (20 mg/mL) Injected every 2.65 minutes |