Columns
BioPro IEX Q/S, which are based on a hydrophilic polymer with low nonspecific adsorption, are designed for capture and intermediate purification of proteins and nucleotides. High dynamic binding capacity (DBC) and high recovery allow fast large-scale purification processes. These media offers high productivity for industrial purification of peptides, proteins, and nucleotides, including biopharmaceuticals such as antibodies.
To BioPro Ion Exchange Screening Kit
| BioPro IEX Q | BioPro IEX S | |
|---|---|---|
| Matrix | Hydrophilic porous polymer | |
| Particle size (μm) | 75 | |
| Charged group | -R-N+（CH3）3 | -R-SO3- |
| Usable pH range | 2.0 - 12.0 | |
| Ion exchange capacity (meq/mL-resin) |
>0.10 | |
| Dynamic binding capacity (DBC) (mg/mL-resin) |
>160 (BSA) | >160 (lysozyme) |
BioPro IEX Q/S have higher DBC for proteins than commercial ion exchange media. BioPro IEX Q/S are effective for protein purification during the capture step.
| Anion exchanger | Particle size (μm) |
Ion exchange capacity (meq/mL-resin) |
DBC (mg/mL-resin) |
|---|---|---|---|
| BioPro IEX Q75 | 75 | 0.13 | 183 |
| Brand G (porous Q type) | 90 | 0.19 | 102 |
| Cation exchanger | Particle size (μm) |
Ion exchange capacity (meq/mL-resin) |
DBC (mg/mL-resin) |
|---|---|---|---|
| BioPro IEX S75 | 75 | 0.12 | 192 |
| Brand G (porous S type) | 90 | 0.13 | 80 |
| Column | ： | 50 X 4.6 mmI.D. |
|---|---|---|
| Linear velocity | ： | 3.0 cm/min (180 cm/hr) |
| Equilibration buffer | ： | 20 mM Tris-HCl (pH 8.6) |
|---|---|---|
| Elution buffer | ： | 0.5 M NaCl in equilibration buffer |
| Sample | ： | 1.5 mg/mL BSA in equilibration buffer |
| Detection | ： | UV at 280 nm |
| Equilibration buffer | ： | 20 mM Glycine-NaOH (pH 9.0) |
|---|---|---|
| Elution buffer | ： | 0.5 M NaCl in equilibration buffer |
| Sample | ： | 1.5 mg/mL Lysozyme in equilibration buffer |
| Detection | ： | UV at 300 nm |
BioPro IEX Q/S show high DBC over a wide range of linear velocities, and the difference in DBC is less than 5% between 200 cm/hr and 1000 cm/hr. BioPro IEX Q/S give increased productivity and reduced cost in biopharmaceutical production.
| Column | ： | 50 X 5.0 mmI.D. |
|---|---|---|
| Equilibration buffer | ： | 20 mM Glycine-NaOH (pH 9.0) |
| Elution buffer | ： | 0.5 M NaCl in equilibration buffer |
| Sample | ： | 1.0 mg/mL Lysozyme in equilibration buffer |
| Detection | ： | UV at 300 nm |
Cleaning in place (CIP) is an important procedure for cleaning and sterilizing columns used for protein purification. The DBC and the selectivity of proteins are unaffected following 20 cycles of CIP with 1 M NaOH. The high chemical stability of BioPro IEX Q/S allow effective cleaning with alkaline solution.
Conditions of DBC measurement
| Column | ： | BioPro IEX S75 50 X 5.0 mmI.D. |
|---|---|---|
| Linear velocity | ： | 800 cm/hr |
| Equilibration buffer | ： | 20 mM Glycine-NaOH (pH 9.0) |
| Elution buffer | ： | 0.5 M NaCl in equilibration buffer |
| Sample | ： | 1.0 mg/mL Lysozyme in equilibration buffer |
| Detection | ： | UV at 300 nm |
※DBC is determined at 10% breakthrough
Egg yolk antibody (IgY) can be isolated with high purity of more than 99% using two chromatographic purification steps, which consist of a capture step using ion exchange chromatography with BioPro IEX Q75 and a polishing step using size exclusion chromatography with YMC-Pack Diol-200.
| Eluent | ： |
A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1) containing 0.5 M NaCl 10%B (0-15 min), 30%B (15-30 min), 90%B (30-40 min) |
|---|---|---|
| Flow rate | ： | 0.5 mL/min (180 cm/hr) |
| Temperature | ： | ambient |
| Detection | ： | UV at 280 nm |
| Injection | ： | 1 mL (ca. 20 mg Protein) |
| Sample | ： | Crude extract from egg yolk (Courtesy of Pharma Foods International Co., Ltd.) |
| Eluent | ： | 0.1 M KH2PO4-K2HPO4 (pH 6.9) containing 0.2 M NaCl |
|---|---|---|
| Flow rate | ： | 0.7 mL/min |
| Temperature | ： | ambient |
| Detection | ： | UV at 280 nm |
| Injection | ： | 1 mL (ca. 0.45 mg IgY) |
| Sample | ： | Fraction from capture step by IEC |
| Packing material | Particle size (μm) |
Product number |
|---|---|---|
| BioPro IEX Q75 | 75 | QAA0S75 |
| BioPro IEX S75 | 75 | SPA0S75 |
| Product name | Particle size (μm) |
Specification | Column volume | Product Number |
|---|---|---|---|---|
| BioPro IEX Q75 | 75 | 5 / pack | 1 mL | BPQAA0S75-01PK |
| 5 mL | BPQAA0S75-05PK | |||
| BioPro IEX S75 | 1 mL | BPSPA0S75-01PK | ||
| 5 mL | BPSPA0S75-05PK |
