YMC-UltraHT Pro C18 is a C18 bonded-phase on highly efficient 2μm spherical silica particles. Since the introduction of the ProFamily series of phases,YMC-Pack Pro C18 has proved to be one of the first choices for a wide range of HPLC applications in pharmaceutical and biotechnological research and production, where efficiency and reliability are a major demand. YMC has transferred these outstanding qualities to ultra-fast LC with the introduction of YMC-UltraHT Pro C18.
Specification
| Particle size | : | 2µm |
|---|---|---|
| Pore size | : | 120Å |
| Carbon contents | : | 16% |
| Usable pH range | : | 2.0-8.0 |
POINT
- First choice ODS column for Ultra-fast LC
- Standard ODS for a wide range of HPLC applications
- Same selectivity and superior peak shapes as 3μm and 5μm Pro C18
- Simple method transfer from conventional HPLC
First choice ODS column for Ultra-fast LC
YMC-UltraHT Pro C18 is designed for high-speed and high-throughput analysis, advanced endcapping technology makes YMC-UltraHT Pro C18 highly appropriate for analysis of a wide range of analytes including basic compounds. This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT Pro C18 column. It can shorten analysis time by 92% without losing the separation characteristics and reduce solvent consumption by 94%.
Antioxidants
- n-Propyl gallate (PG)
- 2,4,5-Trihydroxybutyrophenone (THBP)
- tert-Butylhydroquinone (TBHQ)
- Nordihydroguaiaretic acid (NDGA)
- 4-Hydroxymethyl-2,6-di-tert-butylphenol(HMBP)
- n-Octyl gallate (OG)
- n-Dodecyl gallate (DG)
| Eluent | : |
|
||||
|---|---|---|---|---|---|---|
| Temperature | : | 30ºC | ||||
| Detection | : | UV at 280 nm |
The great separation characteristics and peak shapes by well-known Pro series
During the separation of alkaloids (basic compounds), YMC-UltraHT Pro C18 exhibits great peak shapes with much less tailing compared to commercial silica-based sub-2μm columns.
Alkaloids
- Scopolamine
- Atropine
- Cinchonine
- Quinine
- Dihydroquinine
| Column | : | 50 X 2.0 or 2.1 mmI.D. |
|---|---|---|
| Eluent | : | 20 mM CH3COOH-CH3COONH4 (pH 4.9)/ acetonitrile (80/20) |
| Flow rate | : | 0.6 mL/min |
| Temperature | : | 40ºC |
| Detection | : | UV at 220 nm |
Excellent resolution for a wide range of analytes in Ultra-fast LC
YMC-UltraHT Pro C18 with high-resolution is applicable for high-throughput analysis of samples with complicated matrix such as pharmaceuticals, agricultural chemicals, and foods.
- Gallic acid
- Protocatechuic acid
- Chlorogenic acid
- p-Hydroxybenzoic acid
- Vanillic acid
- trans-Caffeic acid
- Sinapic acid
- p-Coumaric acid
- trans-Ferulic acid
- Salicylic acid
- trans-Cinnamic acid
| Column | : | YMC-UltraHT Pro C18, 2 μm, 50 X 2.0 mmI.D. |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Eluent | : |
|
||||||||
| Flow rate | : | 0.5 mL/min | ||||||||
| Temperature | : | 40ºC | ||||||||
| Detection | : |
|
||||||||