Reversed Phase(ODS)

YMC-UltraHT Pro C18

Columns

YMC-UltraHT Pro C18 is a C18 bonded-phase on highly efficient 2μm spherical silica particles. Since the introduction of the ProFamily series of phases,YMC-Pack Pro C18 has proved to be one of the first choices for a wide range of HPLC applications in pharmaceutical and biotechnological research and production, where efficiency and reliability are a major demand. YMC has transferred these outstanding qualities to ultra-fast LC with the introduction of YMC-UltraHT Pro C18.

Specification

Particle size : 2µm
Pore size : 120Å
Carbon contents : 16%
Usable pH range : 2.0-8.0

POINT

  • First choice ODS column for Ultra-fast LC
  • Standard ODS for a wide range of HPLC applications
  • Same selectivity and superior peak shapes as 3μm and 5μm Pro C18
  • Simple method transfer from conventional HPLC

*To YMC-UltraHT series


First choice ODS column for Ultra-fast LC

YMC-UltraHT Pro C18 is designed for high-speed and high-throughput analysis, advanced endcapping technology makes YMC-UltraHT Pro C18 highly appropriate for analysis of a wide range of analytes including basic compounds. This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT Pro C18 column. It can shorten analysis time by 92% without losing the separation characteristics and reduce solvent consumption by 94%.

First choice ODS column for Ultra-fast LC

Antioxidants

  1. n-Propyl gallate (PG)
  2. 2,4,5-Trihydroxybutyrophenone (THBP)
  3. tert-Butylhydroquinone (TBHQ)
  4. Nordihydroguaiaretic acid (NDGA)
  5. 4-Hydroxymethyl-2,6-di-tert-butylphenol(HMBP)
  6. n-Octyl gallate (OG)
  7. n-Dodecyl gallate (DG)
Eluent :
A) water/TFA (100/0.1)
B) acetonitrile/methanol/TFA (75/25/0.1)
Temperature : 30ºC
Detection : UV at 280 nm

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The great separation characteristics and peak shapes by well-known Pro series

During the separation of alkaloids (basic compounds), YMC-UltraHT Pro C18 exhibits great peak shapes with much less tailing compared to commercial silica-based sub-2μm columns.

The great separation characteristics and peak shapes by well-known Pro series

Alkaloids

  1. Scopolamine
  2. Atropine
  3. Cinchonine
  4. Quinine
  5. Dihydroquinine
Column : 50 X 2.0 or 2.1 mmI.D.
Eluent : 20 mM CH3COOH-CH3COONH4 (pH 4.9)/
acetonitrile (80/20)
Flow rate : 0.6 mL/min
Temperature : 40ºC
Detection : UV at 220 nm

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Excellent resolution for a wide range of analytes in Ultra-fast LC

YMC-UltraHT Pro C18 with high-resolution is applicable for high-throughput analysis of samples with complicated matrix such as pharmaceuticals, agricultural chemicals, and foods.

Excellent resolution for a wide range of analytes in Ultra-fast LC

  1. Gallic acid
  2. Protocatechuic acid
  3. Chlorogenic acid
  4. p-Hydroxybenzoic acid
  5. Vanillic acid
  6. trans-Caffeic acid
  7. Sinapic acid
  8. p-Coumaric acid
  9. trans-Ferulic acid
  10. Salicylic acid
  11. trans-Cinnamic acid
Column : YMC-UltraHT Pro C18,
2 μm, 50 X 2.0 mmI.D.
Eluent :
A) water/HCOOH (200/0.4)
B) acetonitrile/methanol/THF/water/HCOOH
  (43.5/43.5/13/100/0.4)
20-100%B (0-2.8 min)
Flow rate : 0.5 mL/min
Temperature : 40ºC
Detection :
A) UV at 254 nm, B) UV at 280 nm

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