In many cases, the separation of highly polar compounds requires highly aqueous mobile phase conditions to achieve sufficient retention on the stationary phase. The selectivities of conventional C18 do not give reproducible results under these conditions due to mainly collapse of the C18 chains. Therefore, YMC developed Hydrosphere C18 in order of to overcome the loss in retention. Now, this outstanding chromatographic behavior has been transferred to YMC-UltraHT Hydrosphere C18.
Specification
| Particle size | : | 2µm |
|---|---|---|
| Pore size | : | 120Å |
| Carbon contents | : | 12% |
| Usable pH range | : | 2.0-8.0 |
POINT
- The best column for highly polar compounds
- Can be used with 100% aqueous as mobile phase
- Reduced analysis time while maintaining excellent resolution
- Simple method transfer from conventional HPLC
APPLICATION DATA |
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Ultra-fast analysis of highly polar compounds
It is difficult to achieve sufficient retention and resolution of highly polar compounds on conventional C18 phase. YMC-UltraHT Hydrosphere C18 provides superior resolution and excellent reproducibility of those compounds even in ultra-fast LC.
Metabolites of organic solvents in urine (standard mixture)
- Creatinine
- Mandelic acid
- Phenylglyoxylic acid
- Hippuric acid
- o-Methylhippuric acid
- p-Methylhippuric acid
- m-Methylhippuric acid
| Column | : | 50 X 2.0 or 2.1 mmI.D. |
|---|---|---|
| Eluent | : | 20 mM CH3COONH4/IPA (97/3) |
| Flow rate | : | 0.4 mL/min |
| Temperature | : | 35ºC |
| Detection | : | UV at 225 nm |
| Injection | : | 1µL |
Ultra-fast analysis of drug metabolites
YMC-UltraHT Hydrosphere C18 shows stronger retention for the polar metabolite and shorter retention for the hydrophobic unchanged drug substance compared to the other sub-2µm ODS. YMC-UltraHT Hydrosphere C18 is suitable for ultra-fast analysis of drugs and their metabolites that differ greatly in hydrophobicity.
| Column | : | 100 X 2.0 mmI.D. or 100 X 2.1 mmI.D. | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Eluent | : |
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| Detection | : | UV at 220 nm | ||||||||
| Temperature | : | 37ºC | ||||||||
| Injection | : | 1µL | ||||||||
- *Analysis of metabolites on YMC-UltraHT Hydrosphere C18
- Xanthine oxidase inhibitor and its metabolite in human serum
- High sensitive and high throughput analysis of diltiazem metabolites using LC/TOF-MS