Reversed Phase(ODS)
YMC-UltraHT Series
Characteristics of 2µm packing material
YMC-UltraHT series shows the same efficiency as sub-2µm columns for ultra-fast LC system and reduces the system pressure significantly.
| Column | : 50 X 2.0 or 2.1 mmI.D. |
|---|---|
| Eluent | : acetonitrile/water (60/40) |
| Temperature | : 25ºC |
| Sample | : Butyl benzoate |
Reduced analysis time and cost
This example shows the method transfer from conventional HPLC method to Ultra-fast LC method using a YMC-UltraHT column. Since the column efficiency is not decreased even when the linear velocity is 3 times as fast as the conventional condition, it can shorten analysis time by 90% without losing the separation characteristics.
Flavonoids
- Myricetin
- Quercetin
- Apigenin
- Kaempferol
- Baicalein
- Chrysin
- Acacetin
| Column | : acetonitrile/water/formic acid (35/65/0.1) |
|---|---|
| Temperature | : 40ºC |
| Detection | : UV at 260 nm |
Selecting the desired column dimensions
| Feature and Use | For LC/MS or reduction of solvent usage (2.0 mmI.D.) |
For conventional LC system (3.0 mmI.D.) |
|---|---|---|
| Emphasize reduction of analysis time (length: 30 mm) |
30 X 2.0 | - |
| Ultra-fast separation with the similar property as traditional column. (length: 50 mm, 75 mm) |
Standard for Ultra-fast LC 50 X 2.0 |
50 X 3.0 |
| 75 X 2.0 | 75 X 3.0 | |
| High resolution column for closely eluting peaks (length: 100 mm) |
100 X 2.0 | 100 X 3.0 |
Ideal for High-resolution analysis: 100mm column
YMC-UltraHT column can resolve 38% more peaks than a conventional 5µm column with increased sensitivity.
Peptide mapping
| Eluent | : A) water/TFA(100/0.1), B) acetonitrile/TFA(100/0.1) 5-50%B(0-15 min) |
|---|---|
| Flow rate | : 0.4 mL/min |
| Temperature | : 37ºC |
| Detection | : UV at 220 nm |
| Injection | : 1µL |
| Sample | : Tryptic digest of β -Lactogloblin B (5 mg/mL; 37 ºC, 24 hr) |
Easily applicable for conventional LC system: 3.0mmI.D.
YMC-UltraHT series can be used for fast analysis with conventional LC system, as its pressure is lower than commercial sub-2µm column.Considering the extra column band broadening, the column of 3.0 mmI.D.is more applicable for conventional LC system than the column of 2.0 mmI.D.
Cephalosporin antibiotics
- Cephalexin
- Cefaclor
- Cephaloglycin
- Cephaloridine
| Column | : YMC-UltraHT Pro C18 |
|---|---|
| Eluent | : acetonitrile/20mM KH2PO4 (10/90) |
| Temperature | : 37ºC |
| Detection | : UV at 260 nm |