Reversed Phase(ODS)
YMC-Triart C18
Great durability
YMC-Triart C18 shows great chemical stability provided by newly developed hybrid silica. Even in high pH or high temperature conditions, the lifetime of YMC-Triart C18 is 10 times greater than conventional ODS columns.
Durability in high pH
Phosphate buffer(pH 11.5),40ºC
Triethylamine(pH 11.5),40ºC
Durability in high temperature
pH 6.9, 70ºC
pH 1, 70ºC
Flexibility in method development
YMC-Triart C18 may be used over a pH range of 1~12, enabling the method development chemist great flexibility to manipulate the pH of the eluent to affect optimal retention and resolution of analysis.
Adjust pH and/or choice of organic solvent to adjust resolution and retention
- Saccharin
- Dextromethorphan
- Amitriptyline
- n-Butylparaben
- Ibuprofen
Excellent resolution
YMC-Triart C18's innovative surface modification technology makes for excellent peak shapes for all types of compounds.
Without adsorption and tailing
Excellent reproducibility
YMC-Triart C18 exhibits excellent lot to lot reproducibility for all types of compounds including basic and coordination compound, which are difficult to chromatograph.
Basic compounds
- Chlorpheniramine
- Dextromethorphan
- Propyl p-hydroxybenzoate
| Column | : | 5 μm, 150 X 3.0 mmI.D. |
|---|---|---|
| Eluent | : | 20 mM KH2PO4-K2HPO4 (pH 6.9)/ acetonitrile (65/35) |
| Flow rate | : | 0.425 mL/min |
| Temperature | : | 40ºC |
| Detection | : | UV at 235 nm |
Coordination compounds
- Hinokitiol
- Methyl benzoate
| Column | : | 5 μm, 150 X 3.0 mmI.D. |
|---|---|---|
| Eluent | : | acetonitrile/0.1% H3PO4 (40/60) |
| Flow rate | : | 0.425 mL/min |
| Temperature | : | 40ºC |
| Detection | : | UV at 254 nm |
Excellent versatility
Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C18 can separate clemastine sharply using many buffer/solvent combinations.
Comparison of clemastine analysis with conventional columns
Clemastine
| Column | : | 5 μm, 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D. | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Eluent | : |
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| Flow rate | : | 0.2 mL/min | ||||||||
| Temperature | : | 25ºC | ||||||||
| Detection | : | UV at 230 nm | ||||||||
Reduced column bleed
Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection techniques. In the example cited below, use of a 2 x 50 mm YMC-Triart C18 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques that are sensitive to particle degradation.
Bleed test by LC/MS
| Column | : | 5 μm, 50 X 2.0 mmI.D. | ||||||||
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| Eluent | : |
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| Flow rate | : | 0.4 mL/min | ||||||||
| Temperature | : | 40ºC | ||||||||
| Detection | : | ESI positive, TIC (Mass Range: 50-1000) | ||||||||
Minimizing strong solvent/sample loading effects
YMC-Triart C18 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of organic solvent for solubilization prior to analysis.
Influence on peak shapes by injection volume
| Column | : | 5 μm, 50 X 2.0 mmI.D. or 2.1 mmI.D. | ||||||
|---|---|---|---|---|---|---|---|---|
| Eluent | : |
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| Flow rate | : | 0.4 mL/min | ||||||
| Temperature | : | 40ºC | ||||||
| Detection | : | UV at 275 nm | ||||||
Low back-pressure
YMC-Triart C18 is designed for use in all conditions. Even with higher viscosity methanol, YMC-Triart C18 can operate with lower pressure (typically 30% less than conventional columns).
Relationship between solvents and column pressure
| Column | : | 5 μm, 150 X 4.6 mmI.D. |
|---|---|---|
| Eluent | : | acetonitrile/water or methanol/water |
| Flow rate | : | 1.0 mL/min |
| Temperature | : | 25ºC |