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YMC-Pack PVA-Sil

YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external and internal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. The PVA polymer shell on PVA-Sil deactivates the silica support while roviding a hydrophilic surface.

SPECIFICATION

Particle size : 5 µm
Pore size : 120 Å
Usable pH range : 2.0-9.5
Recommended pH range : 2.0-7.5

POINT

  • Vinyl alcohol polymerised silica
  • High stability and reproducibility
  • Excellent choice for SFC (Supercritical Fluid Chromatography)

Application

Potato Lipids

(Literature: W.W. Christie; R.A. Urwin, J. High Resol. Chromatogr., Vol. 18 (1995) P97 - 100)
Column : YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D.
Flow rate : 1 to 2 mL/min
Eluent : A) 2-methylpentane/methyl tert-butyl ether (98/2)
B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025)
C) 2-propanol/water/triethylamine (50/50/0.2)
Gradient :
Tmin : 0 5 15 40 40.1 45 50
B% : 0 20 52 52 70 0 0
C% : 0 0 4 14 0 0 0
Flow (mL/min) : 1 1 1 1.4 1.4 2 2
Nebuliser temperature : 25ºC, Evaporation temperature : 35ºC
Detector : ELSD
Sample :
S : Sterols CER : Cerebrosides
SE : Sterol Esters SG : Steryl glycosides
MGDG : Monogalactosyldiacylglycerols DGDG : Digalactosyldiacylglycerols
PE : Phosphatidylethanolamine PG : Phosphatidyl glycerols
PC : Phosphatidylcholine ASG : Acylsterylglycosides
PI : Phosphatidylinositol DG : Diacylglycerol
Nucleotides (in HILIC mode)
Column : YMC-Pack PVA-Sil (5 μm, 120 Å)
150 x 2.0 mmI.D.
Eluent : acetonitrile/100 mM CH3COONH4 (75/25)
Flow rate : 0.2 mL/min
Temperature : 40ºC
Detection : UV at 254 nm

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