Columns
YMC-Pack PVA-Sil, bonded with a monomolecular polymer coating of vinyl alcohol (PVA), completely covers both external and internal surfaces of the silica support, protecting it against aggressive, high pH buffers and solvents. The PVA polymer shell on PVA-Sil deactivates the silica support while roviding a hydrophilic surface.
Particle size | : 5 µm |
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Pore size | : 120 Å |
Usable pH range | : 2.0-9.5 |
Recommended pH range | : 2.0-7.5 |
Column | : | YMC-Pack PVA-Sil (5 μm, 120 Å), 250 × 4.6 mmI.D. | ||||||||||||||||||||||||||||||||||||||||||||
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Flow rate | : | 1 to 2 mL/min | ||||||||||||||||||||||||||||||||||||||||||||
Eluent | : | A) 2-methylpentane/methyl tert-butyl ether (98/2) B) 2-propanol/acetonitrile/CHCl3/CH3COOH (84/8/8/0.025) C) 2-propanol/water/triethylamine (50/50/0.2) |
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Gradient | : |
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Nebuliser temperature | : | 25ºC, Evaporation temperature : 35ºC |
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Detector | : | ELSD | ||||||||||||||||||||||||||||||||||||||||||||
Sample | : |
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Column | : | YMC-Pack PVA-Sil (5 μm, 120 Å) 150 x 2.0 mmI.D. |
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Eluent | : | acetonitrile/100 mM CH3COONH4 (75/25) |
Flow rate | : | 0.2 mL/min |
Temperature | : | 40ºC |
Detection | : | UV at 254 nm |