Columns

YMC-Pack Pro C8&C4

All phases in the Pro-family are ideal for the analysis of all types of organic molecules but specifically basic and metal chelating compounds. The ultrapure silica and very low residual silanol activity means that these columns frequently do not require the use of ion-pairing reagents or ion-suppression techniques, making them ideal for LC/MS applications.

SPECIFICATION
Particle size 3 µm, 5 µm
Pore size 120 Å
Carbon contents C8--10%
C4--7%
Usable pH range 2-7.5
POINT
  • Consistently reproducible quality
  • Documentation of each manufacturing step
  • Consistently high packing quality + column efficiency
  • Stringent specifications
  • Individual test of each column (test chromatogram)
  • Batch identification for each column
Features

Separation of basic compounds

Effectively endcapped Pro C8 and C4 offer better peak shape for separation of basic compounds such as alkaloids.

  1. Scoporamine bromide
  2. Atropine sulfate
  3. Cinchonin
  4. Quinine
  5. Papaverine chloride
Column YMC-Pack Pro C8 (5 µm, 120 Å)
150 x 4.6 mmI.D.
Eluent acetonitrile/20 mM KH2PO4 (20/80)
Flow rate 1.0 mL/min
Temperature 30°C
Detection UV at 210 nm

Short time analysis

This figure shows that Pro C8 and C4 columns allow us to minimize total analysis time while maintaining excellent separation for caffeine and ethenzamide and bromovalerylurea.

  1. Caffeine
  2. Ethenzamide
  3. Bromovalerylurea
  4. Ibuprofen
Column 150 x 4.6 mmI.D.
Eluent methanol/20 mM H3PO4 (60/40)
Flow rate 1.0 mL/min
Temperature 37°C
Detection UV at 254 nm

Optimization of separation by Pro C8 and C4

In the case that it is difficult to optimize resolution and analysis time on separation by C18, C8 and C4 columns can be effective alternatives.

  1. Phenytoin
  2. Propranolol HCI
  3. Quinidine
  4. Lidocaine
  5. Diltiazem HCI
  6. Verapamil HCI
  7. Nicardipine HCI
Column 150 x 4.6 mmI.D.
Eluent 20 mM KH2PO4-K2HPO4 (pH6.9) /methanol
Flow rate 1.0 mL/min
Temperature 37°C
Detection UV at 220 nm