Columns
CHIRAL ART provide good peak shapes for ionic and metal coordination compounds.
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/2-propanol/diethylamine (80/20/0.1) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 230 nm |
| Injection | 10 μL (0.1 mg/mL) |
CHIRAL ART Immobilized type show remarkably reduced background signals under typical gradient conditions. The low column bleeding of these columns provides high sensitivity for LC/MS analysis owing to the very low ion suppression and stable baseline. CHIRAL ART Immobilized type offers excellent robustness for gradient analysis and highly sensitive analysis on LC/MS.
| Gradient test | |
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | A) n-hexane B) ethanol 2-80%B (0-30 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 230 nm |
CHIRAL ART Immobilized type has excellent chemical stability and can be used across a wide pH range. The addition of acid/amine to the mobile phase or the use of buffer solution is an effective tool for improving peak shape and/or resolution.
| Continuous flow of acid/alkaline solution | |
| Column | 5 μm, 50 X 4.6 mmI.D. |
|---|---|
| Eluent | Buffer/methanol (90/10) |
| Flow rate | 1.0 mL/min |
| 【Acidic condition】 | |
| Buffer | 0.1% H3PO4 (pH 2) |
| Temperature | 40°C |
| 【Basic condition】 | |
| Buffer | 20 mM NH4HCO3-DEA (pH 9) |
| Temperature | 25°C |
| Column performance test | |
| Column | 5 μm, 50 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water (30/70) for SB methanol/water (70/30) for SJ |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 254 nm |
| Sample | Benzoin for SB 1-(1-Naphthyl) ethanol for SJ |
On CHIRAL ART Immobilized type, the change in column performance after flushing with each solvent was less than 2%. Thus, these columns have high resistance to various solvents.
| Amylose-SA | Cellulose-SB | Cellulose-SJ | ||||
|---|---|---|---|---|---|---|
| α | k'(2) | α | k'(2) | α | k'(2) | |
| Ethyl acetate | 100.3% | 101.2% | 100.0% | 99.1% | 99.3% | 99.0% |
| Tetrahydrofuran | 100.0% | 100.0% | 99.3% | 98.0% | 99.2% | 99.7% |
| Dichloromethane | 100.3% | 100.6% | 101.3% | 99.6% | 99.6% | 98.4% |
| Column | 5 μm, 50 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/2-propanol (95/5) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Sample | Benzoin |
