Columns
CHIRAL ART provide good peak shapes on ionic and metal coordination compounds.
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/2-propanol/diethylamine (80/20/0.1) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 230 nm |
| Injection | 10 μL (0.1 mg/mL) |
CHIRAL ART have outstanding packed bed stability provided by the use of high-strength super-wide-pore silica and innovative packing technology. The column efficiency and selectivity are maintained, even after sequential gradient tests at a high flow rate (three times higher than the normal flow rate) and a high pressure (rapid pressure change).
CHIRAL ART are suitable for the case where one intends to shorten an analysis time, (re-)equilibration time, and/or column cleaning time by increasing the flow rate. This feature is also effective when a highly viscous solvent needs to be used as a mobile phase on immobilized type columns.
| Sequential gradient test | |
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | A) n-hexane, B) ethanol 0-100%B (0-15 min) |
| Flow rate | 3.0 mL/min |
| Pressure | 10-30 MPa/run |
| Temperature | 37℃ |
| Column performance test | |
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/ethanol (90/10) |
| Flow rate | 1.0 mL/min |
| Temperature | 37℃ |
| Detection | UV at 230 nm |
| Sample | trans-Stilbene oxide |
On both analytical and preparative separation, separation behavior of CHIRAL ART is equivalent to that of the competitor’s product.
| Column | 5 μm, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/ethanol (90/10) |
| Flow rate | 1.0 mL/min |
Ibuprofen enantiomers were purified by utilizing the recycling mode of a multiple preparative HPLC system, LC-Forte/R. Recycling chromatography is effective when method optimization for chiral isolation is difficult using single column separation. The recycling method offers high-purity and high-recovery purification. Furthermore, no solvent is consumed during recycling, which greatly contributes to the reduction of solvent consumption during purification.
| Column | CHIRAL ART Cellulose-C 10 µm, 250 X 20 mmI.D. |
|---|---|
| Flow rate | 20 mL/min |
| Detection | UV at 265 nm |
| Injection | 5 mL (10 mg/mL) |
| Single Column | Recycling | |
| Enantiomeric purity (%ee ) Fr.1 (R)(- -)-Ibuprofen Fr.2 (S)(- +)-Ibuprofen |
95.0 96.8 |
98.4 99.2 |
| Yield (%) | 84 | 95 |
| Solvent consumption (mL solvent/g product) |
9,523 | 1,276 |
