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Alcyon SFC

Alcyon SFC columns are Supercritical Fluid Chromatography (SFC) columns available with chiral/achiral stationary phases. Alcyon SFC columns are specifically packed in SFC-compatible hardware and are tested under SFC conditions.
The low viscosity of supercritical carbon dioxide allows for analytical separations that are 3–5 times faster than those for normal-phase HPLC.

Catalog download pdf

Application of SFC

POINTS

  • Available in chiral and achiral stationary phases
  • Faster separation with high resolution
  • Excellent durability
  • Great reduction of solvent consumption


Chiral and achiral stationary phases

[ Chiral Immobilized type ]

 
Particle size
(µm)
Chiral selector
Column size*
inner diameter X length (mm)
Alcyon SFC CSP Amylose-SA 3
5
2.1 X 150
3.0 X 50
3.0 X 100
3.0 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
Alcyon SFC CSP Cellulose-SB
Alcyon SFC CSP Cellulose-SC
Alcyon SFC CSP Cellulose-SJ 5 2.1 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250

* See Product List for details of column size lineup.

[ Chiral Coated type ]

 
Particle size
(µm)
Chiral selector
Column size*
inner diameter X length (mm)
Alcyon SFC CSP Amylose-C 3
5
2.1 X 150
3.0 X 50
3.0 X 100
3.0 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
Alcyon SFC CSP Cellulose-C

* See Product List for details of column size lineup.

[ Achiral ]

 
Particle size
(µm)
Functional group
Column size
inner diameter X length (mm)
Alcyon SFC Triart C18
5
-C18H37
2.1 X 150
4.6 X 150
4.6 X 250
Alcyon SFC Triart Diol
Alcyon SFC Triart PFP
Alcyon SFC CN
-CH2CH2CH2CN
2.1 X 150
4.6 X 150
4.6 X 250
10 X 250
20 X 250
Alcyon SFC SIL
(-OH)

 

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Faster separation with high resolution

Faster chiral separation of trans-Stilbene oxide is achieved using supercritical fluid chromatography compared with HPLC separation. The lower viscosity and larger diffusion coefficient of the supercritical fluid provide rapid separation of both chiral and achiral compounds.

[ SFC condition ]
Column Alcyon SFC CSP Amylose-C
5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (60/40)
Flow rate 3.0 mL/min
Temperature 40ºC
Detection UV at 220 nm
Back pressure 17.2 MPa (2500 psi)
[ HPLC condition ]
Column CHIRAL ART Amylose-C
5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (90/10)
Flow rate 1.0 mL/min
Temperature 25ºC
Detection UV at 220 nm

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Excellent peak shape ~Good peak shape in mobile phase without the addition of acid~

Excellent peak shape of 2-Phenylpropionic acid is obtained using SFC chiral separation. Under HPLC conditions, the peak shape is very broad with mobile phases containing no additives, such as acid. However, using SFC, the peak shapes are very good with just a mixture of CO2 and methanol. Supercritical carbon dioxide is considered to act as an weak acid.

 

[ SFC condition ]
Column Alcyon SFC CSP Cellulose-C
5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (98/2)
Flow rate 3.0 mL/min
Temperature 35ºC
Detection UV at 220 nm
Back pressure 10.3 MPa (2000 psi)
[ HPLC condition ]
Column CHIRAL ART Cellulose-C
5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (99/1)
Flow rate 1.0 mL/min
Detection UV at 220 nm

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Selectable column to suit the sample characteristics

The results of analyzing phenols on three kinds of achiral columns are shown. As the separation behavior is different, a column can be selected to suit the sample characteristics.
YMC offers five achiral-phase SFC columns.

 

[ Alcyon SFC Triart Diol ]

[ Alcyon SFC SIL ]

[ Alcyon SFC CN ]

Column 5 µm, 250 X 4.6 mmI.D.
Eluent CO2/methanol (88/12)
Flow rate 3.0 mL/min
Temperature 30ºC
Detection UV at 230 nm
Back pressure 10.3 MPa (2000 psi)

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SFC preparative separation of flavanone

Alcyon SFC columns show excellent peak shape, even for faster separations. As a result, high-purity and high-recovery purification will be achieved. Alcyon SFC columns offer purification with higher efficiency owing to lower solvent consumption.

SFC Fr.1 Fr.2
Enantiomeric purity >99.9 %ee 99.8 %ee
Yield 94.5 % 95.6 %
Productivity*

340
(mg product/hr)

344
(mg product/hr)

Column Alcyon SFC CSP Amylose-C
5 µm, 250 X 20 mmI.D.
Eluent CO2/ethanol (80/20)
Flow rate 60 mL/min
Temperature 30ºC
Detection UV at 280 nm
Back pressure 15 MPa (2180 psi)
Injection 1.5 mL (20 mg/mL)

*Calculated based on injections of every 2.5 minutes on SFC.

  • Cost effective purification using SFC
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    Excellent durability

    « High column durability under repeated backpressure gradient conditions »

    The results of sequential analysis under backpressure gradient conditions on Alcyon SFC CSP Amylose-C are shown.

    The theoretical plate number and retention time are maintained, even after the sequential gradient test. The Alcyon SFC column has excellent durability under such tough conditions.

    Change rate of theoretical plate number
    Change rate of retention time
    Durability test
    Column Alcyon SFC CSP Amylose-C
    5 µm, 250 x 4.6 mmI.D.
    Eluent CO2/methanol (80/20)
    Flow rate 1.0 mL/min
    Temperature 50ºC
    Back pressure 10.3 -24.1 MPa (1500-3500 psi) (0-10 min)
    10.3 MPa (1500 psi) (10-13 min)
    Column performance test (every 20 gradient cycles)
    Column Alcyon SFC CSP Amylose-C
    5 µm, 250 x 4.6 mmI.D.
    Eluent CO2/methanol (80/20)
    Flow rate 3.0 mL/min
    Temperature 50ºC
    Detection UV at 220 nm
    Back pressure 10.3 MPa (1500 psi)
    Sample trans-Stilbene oxide

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